BMP-2对人卵巢浆液性囊腺癌HO8910细胞凋亡的影响及机制研究  

Influence of bone morphogenetic protein-2 on the apoptosis of HO8910 ovarian cancer cells

作　　者：马颖[1] 郭权[1] 马琳[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2010年第12期2333-2338,共6页Journal of Modern Oncology

摘　　要：目的:研究BMP-2对人卵巢浆液性囊腺癌HO8910细胞的影响及其机制。方法:MTT、流式细胞仪技术、Annexin V/PI双染、Hoechst 33258染色观察BMP-2对HO8910细胞凋亡的影响,并检测BMP-2作用后HO8910细胞中p21、caspase-3的变化。结果:BMP-2作用后HO8910细胞生长被抑制,凋亡增加,且p21、caspase 3的表达较前增强。结论:BMP-2促进HO8910卵巢癌细胞凋亡,抑制其生长。Objective：To study the influence of bone morphogenetic protein-2（BMP-2） on the apoptosis of human ovarian serous cystadenoma cell HO8910,and to determine the possible mechanism.Methods：MTT,flow cytometry,Annexin V/PI double stain and Hoechst 33258 stain were applied to observe the growth and apoptosis of HO8910 cells after BMP-2 intervention.p21 and caspase-3 of HO8910 cells were observed following BMP-2 intervention.Results：Following the BMP-2 intervention,the growth of HO8910 cells was inhibited,and the apoptosis increased.Both p21 and caspase 3 expressions were boosted.Conclusion：BMP-2 can promote the apoptosis of HO8910 cells and inhibit their growth.

关 键 词：卵巢癌 BMP-2 凋亡 P21 CASPASE 3 

分 类 号：R737.31[医药卫生—肿瘤]