白术碱溶性多糖提取工艺研究  

Study on the Extraction Technology of Alkali-soluble Polysaccharides from Atractylodes macrocephala Koidz

作　　者：贺亮[1,2] 吴学谦[1,2] 程俊文[1] 李海波[1] 付立忠[1] 吴庆其[1,2] 魏海龙[1] 吴大丰 胡传久[1] 

机构地区：[1]浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室,浙江杭州310023 [2]浙江省丽水市食用菌研究开发中心,浙江丽水323000 [3]浙江益圣菌物发展有限公司,浙江丽水323000

出　　处：《安徽农业科学》2010年第33期18777-18779,共3页Journal of Anhui Agricultural Sciences

基　　金："948"项目(2008-4-64);浙江省省院合作项目(2008SY02);"863"计划项目(2007AA10Z339);火炬计划项目(2008GH020685);浙江省创新团队建设与人才培养项目(2009F20019);浙江省林业科技项目(07A07);浙江省林业科研成果推广项目(08A07)

摘　　要：[目的]优化白术(Atractylodes macrocephala Koidz)碱溶性多糖提取工艺。[方法]以白术水提残渣为原料,采用碱溶液进行提取。在单因素试验基础上,选取碱浓度、提取时间和料液比进行正交试验,对其碱溶性多糖的最佳提取工艺进行探讨。[结果]碱溶性白术多糖的最佳提取工艺条件为:碱浓度0.9 mol/L,提取时间5 h,料液比1∶30(W∶V)。在该工艺条件下,碱溶性粗多糖得率为8.38%。[结论]该研究可为白术碱溶性多糖的开发利用提供科学依据。[Objective]The research aimed to optimize the extraction technology of alkali-soluble polysaccharides from Atractylodes macrocephala Koidz.[Method]Using Atractylodes macrocephala Koidz as raw material,the optimal extraction technologies of alkali-soluble polysaccharides were probed and the precipitation properties of the polysaccharides were studied on the basis of single factor and orthogonal experiment.[Result]The results showed that the best technology of extracting alkali-soluble polysaccharides from Atractylodes macrocephala Koidz was as follow∶The concentration of NaOH 0.9 mol/L,time 5 h,the ratio of material to NaOH was 1∶30.Under this condition,the extracting rate of alkali-soluble polysaccharides was 8.38%.[Conclusion] The study can provide scientific basis for development and utilization of alkali-soluble polysaccharides from Atractylodes macrocephala Koidz.

关 键 词：白术 碱溶性多糖 提取工艺 正交试验 

分 类 号：S567.233[农业科学—中草药栽培]