巨齿蛇毒发色底物法检测血浆凝血酶原活性在肝病诊断中的应用  

Application of deception of Plasma facer Ⅱ coagulant activity by echis carinatus venom chromogenic substrateSubstray in the diagnosis of hepatic desease

作　　者：张吉林[1] 宋玉国[2] 赵琦 

机构地区：[1]吉林医学院生物化学考研室,吉林市132001 [2]吉林医学院附属医院 [3]吉林医学院康复医院

出　　处：《中国实验诊断学》1999年第2期73-74,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科委

摘　　要：评估血浆凝血酶原活性（ＦⅡ：ＣＡ）在肝病诊断中的应用价值。方法采用巨齿蛇毒发色底物法（ＥＣＶＣＳＡ）检测了１００例献血员和４００例各型肝炎的ＦⅡ：ＣＡ。结果ＦⅡ：ＣＡ正常参考范围为（８５．０～１３１．０）％；急性肝炎、慢性肝炎轻度和肝硬化Ａ级为（８４．０～７２．５）％，轻度下降；慢性肝炎中、重度和肝硬化Ｂ级为（７２．４～５４．６）％，中度下降；重症肝炎和肝硬化Ｃ级为（５４．５～２５．９）％，重度下降。当ＦⅡ：ＣＡ低于３０％，肝病患者存活率为４．０％，而高于５０％存活率为９２．９％。结论在肝病中对ＦⅡ：ＣＡ的测定可估计病情、评价疗效和判断预后。<Abstratct> To evaluate application of plasma factor II coagulant activity (FII:CA) in the diagnosis of hepatic disease.Mdses Echis carinatus venon chromogenic substrate assay (ECVCSA) was in the detection of FII:CA in theplasma Of 100 health subjects and in 400 patients with eifferent types of hepatitis.Results normal FII:CA referencelevels were (85.0~131.0)%,but in acute hepatitis,chronic mild hepatitis and livereirrhosis,A grade was (84.0~72.5)%;it belongs to slight descent .In chronic middle or serious hepatitis and livercirrhosis,B grade was (72.4~54.6)%;it refers moderate descent. And in serious hepatitis and liveroirrhosis,C grade was (54.5~25.9)%;it belongs to going down seriously.Sur-vival rates of the patients suffered from hepatic disease were 4.0% when F II:CA was lower than 30%,but the survivalrates were 92.9% while FII:CA was higher than 50%.Conclnsion The measurment of FII:CA. may be used to assessthe state of hepatic disease, evaluate curative effect and make prognosis in the hepatic disease.

关 键 词：凝血酶原 巨齿蛇毒 发色底物法 肝病 诊断 

分 类 号：R575.04[医药卫生—消化系统] R446.112[医药卫生—内科学]