基于微生物同化作用的D-丙氨酸生产工艺研究  

Research on D-Alanine Production Technology Based on Microbial Assimilation

作　　者：蒋光玉 章晖 刘洋 唐思青 郑向辉 刘迎伟 

机构地区：[1]安徽华恒生物工程有限公司,合肥231131

出　　处：《氨基酸和生物资源》2010年第4期41-45,共5页Amino Acids & Biotic Resources

基　　金：科技型中小企业技术创新基金重点项目(No.09C26213404268)

摘　　要：以L-丙氨酸为唯一碳氮源,从采集的若干土壤中初筛出能够降解L-丙氨酸的菌株;再以D-丙氨酸为唯一碳氮源,复筛出降解L-丙氨酸而不降解D-丙氨酸的菌株。依据菌种对DL-丙氨酸的不对称降解活性,筛选出具有最高的L-丙氨酸降解活性的菌株,并对菌株同化L-丙氨酸的反应条件进行了研究。结果表明:编号为ALA-D82的菌株具有最高的降解L-丙氨酸的能力,经鉴定为酵母菌属。在30℃,控制pH6.0,通气比1:1(V/V)和转速900 r.min-1的条件下,L-丙氨酸降解的速度最大。在最适条件下,1500 g DL-丙氨酸分两部分添加入7 L的反应液中。反应72 h后溶液中的L-丙氨酸被完全降解,提取得到D-丙氨酸晶体,产率和光学纯度分别达到92.13%和99%。The strains degrading L-alanine were screened from soil samples collected in several districts.L-alanine was used as sole carbon and nitrogen sources.Then D-alanine was used as sole carbon and nitrogen sources,other strains disabling degradation of D-alanine were screened from the first screened strains.Based on activity of the asymmetric degradation of DL-alanine,the strains with the highest activity of L-alanine degradation were selected,and L-alanine assimilation reaction conditions were studied.Results showed that： the strain named ALA-D82 had the highest ability to degrade L-alanine,and was identified as Saccharomyces.At 30 ℃,pH6.0,ventilation ratio 1：1（V / V） and 900rpm of speed,the degradation rate of L-alanine was maximum.Under the optimum conditions,1500g DL-alanine was divided into two parts and added into 7L reaction solution.After 72h the L-alanine in reaction solution was completely degraded,and D-alanine crystals extracted with the yield of 92.13%and optical purity of 99%.

关 键 词：D-丙氨酸 DL-丙氨酸 酵母菌 同化作用 降解反应 

分 类 号：Q93[生物学—微生物学]