壳聚糖微球吸附交联法固定β-葡萄糖醛酸苷酶  

Immobilization of β-glucuronidase on Chitosan Beads by Adsorption and Crosslinking

作　　者：孙倩[1] 李开雄[1] 李春[2] 段海燕[2] 叶海[2] 曹红[2] 

机构地区：[1]石河子大学食品学院,石河子832000 [2]石河子大学化学化工学院,石河子832000

出　　处：《食品工业》2010年第6期13-16,共4页The Food Industry

基　　金：教育部科学技术研究重点项目"固定化β-葡萄糖醛酸苷酶在离子液体中催化合成GAMG的研究"(209146);兵团博士资金项目"离子液体中酶法合成GAMG新工艺的研究"(2009JC13);2008年度石河子大学高层次人才科研启动资金专项"酶膜生物反应器制备GAMG的研究"(RCZX200805)

摘　　要：以壳聚糖微球为载体,采用吸附-交联法将β-葡萄糖醛酸苷酶固定化,采用正交试验设计最佳固定化条件,β-葡萄糖醛酸苷酶的最佳固定化条件为:壳聚糖浓度2.0%、吸附时间12 h、戊二醛浓度0.58%、交联温度40℃、交联时间3 h,固定化酶的酶活回收率最高,可达到74.10%。β-glucuronidase was immobilized on chitosan beads by adsorption and crosslinking. The method of orthogonal test was used to investigate the optimal condition of immobilization. The optimal conditions of immobilization were achieved, when chitosan concentration was 2.0%, and adsorption time was 12 h, followed by crosslinking at 40℃ for 3 h in 0.58% glutaraldehyde solution. The recovery rate of immobilized β-glucuronidase activity was 74.10%.

关 键 词：Β-葡萄糖醛酸苷酶 壳聚糖 固定化 

分 类 号：Q814.2[生物学—生物工程]