猪IGF2 Intron3 G3072A突变及其在背最长肌中差异表达  

The mutation in insulin-like growth factor 2 gene Intron3 G3072A and the difference of IGF2 mRNA expression in longissimus dorsi muscle in pig

作　　者：虞德兵[1] 熊锴[1] 张宇霞[1] 苇小菲 文平[1] 王林云[1] 黄瑞华[1] 刘红林[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2010年第6期105-109,共5页Journal of Nanjing Agricultural University

基　　金：教育部高等学校博士点基金项目(200803071048);南京农业大学青年科技创新基金项目(KJ09013);国家863计划项目(2007AA10Z149)

摘　　要：采用错配PCR-RFLP法检测胰岛素样生长因子2基因(IGF2)内含子3的G3072A位点变异对长×大杂交猪肌肉生长和脂肪沉积的影响,并采用实时荧光定量PCR法分析该变异对出生后的长×大杂交后代猪背最长肌中IGF2mRNA表达发育性变化的影响。结果显示:IGF2内含子3的G3072A位点变异(G→A)对猪背膘厚和活体瘦肉率均存在显著或极显著影响,表现为遗传父本A等位基因的个体能够增加活体瘦肉率和减少背膘厚;在长×大杂交后代的背最长肌中,IGF2mRNA的表达量在遗传父本A等位基因的个体中显著高于遗传父本G等位基因的个体,在42和180日龄时达显著水平(P<0.05),而在70、90和120日龄时达极显著水平(P<0.01)。A mispairing PCR was used to flank the paternal expressed insulin-like growth factor 2 （ IGF2） gene Intron3 G3072A mutation,which was subjected to identify the effect of the variation on muscle developing and fat deposition in LanDrance × Large White cross,and the influence of the variation on the developing changes in longissimus dorsi muscle via real-time PCR. The results showed that the single nucleotide polymorphism in IGF2 gene intron 3 G3072A site had an important or very important influence on muscle growth and fat deposition. Compared with the boars inherited from parental G allele,the IGF2 gene expression in the boars inherited from parental A allele exhibited remarkable up-regulation at day 42 and 180 （ P〈 0. 05） ,respectively,and as significanty up-regulation at day 70,90 and 120 （ P〈 0. 01） ,respectively,in longissimus dorsi muscle in LanDrance × Large White cross.

关 键 词：猪 IGF2基因 瘦肉率 背膘厚 背最长肌 

分 类 号：S852.1[农业科学—基础兽医学] Q786[农业科学—兽医学]