Highly sensitive fluorescence quantification of irinotecan in biological fluids with the aid of second-order advantage  

作　　者：Hai Yan Fu Hai Long Wu Jin Fang Nie Yong Jie Yu Hong Yan Zou Ru Qin Yu 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

出　　处：《Chinese Chemical Letters》2010年第12期1482-1486,共5页中国化学快报（英文版）

基　　金：supported by The National Natural Science Foundation of China(No.20775025);The National Basic Research Program(No.2007CB216404) as well as PCSIRT.

摘　　要：A rapid,green and highly sensitive excitation-emission matrix（EEM） fluorescence method was proposed for analysis of irinotecan（CPT-11） in biological fluids including human plasma and urine samples of uncalibrated interferences with the aid of second-order advantage.Due to the serious spectral overlapping from biological matrices,the parallel factor analysis（PARAFAC） and the alternating normalization-weighted error（ANWE） have been recommended to perform directly calibration and overcome the problem which makes the traditional fluorospectrophotometer in trouble.Satisfactory results can be achieved.Furthermore, performance of the proposed method was evaluated based on figures of merit and some statistical parameters.The accuracy of both algorithms was validated by the elliptical joint confidence region（EJCR） test.The precision and repeatability were also investigated by the relative standard deviations（RSDs） of intra-day and inter-day.A rapid,green and highly sensitive excitation-emission matrix（EEM） fluorescence method was proposed for analysis of irinotecan（CPT-11） in biological fluids including human plasma and urine samples of uncalibrated interferences with the aid of second-order advantage.Due to the serious spectral overlapping from biological matrices,the parallel factor analysis（PARAFAC） and the alternating normalization-weighted error（ANWE） have been recommended to perform directly calibration and overcome the problem which makes the traditional fluorospectrophotometer in trouble.Satisfactory results can be achieved.Furthermore, performance of the proposed method was evaluated based on figures of merit and some statistical parameters.The accuracy of both algorithms was validated by the elliptical joint confidence region（EJCR） test.The precision and repeatability were also investigated by the relative standard deviations（RSDs） of intra-day and inter-day.

关 键 词：IRINOTECAN Biological fluids Excitation-emission matrix fluorescence PARAFAC ANWE 

分 类 号：O175.1[理学—数学] Q592[理学—基础数学]