核桃隔膜多糖的分离纯化及单糖组成分析  

Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.

作　　者：高莉[1] 王强[2] 帕提古力.玛合木提 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]新疆大学理化性质测试中心,新疆乌鲁木齐830046

出　　处：《食品科学》2010年第21期182-185,共4页Food Science

摘　　要：目的:分离纯化核桃隔膜多糖并分析其单糖组成。方法:核桃隔膜经水提醇沉、冷冻干燥得水溶性多糖,经DEAE-纤维素52离子柱和SephadexG-100凝胶柱分离纯化后得到多糖组分PDJL-A11(diaphragma of juglans regia L.acid polysaccharide)。运用紫外光谱鉴定纯度,气相色谱分析多糖的单糖组成。结果:核桃隔膜多糖PDJL-A11单糖组成为:阿拉伯糖、果糖、甘露糖、鼠李糖和葡萄糖,其物质的量比为2.11:6.52:8.81:12.59:15.58。结论:建立了核桃隔膜多糖分离纯化的方法,确定了其单糖组成及其物质的量比、可为核桃隔膜药理学的研究提供参考。The purpose of this work was to isolate and purify polysaccharide from diaphragma of Juglans regia L（PDJL） and analyze its monosaccharide composition.A water-soluble crude polysaccharide was obtained by water extraction,ethanol precipitation and freeze-drying.The crude polysaccharide was purified to obtain a fraction of PDJL-A11 on DEAE-cellulose52 column and SephadexG-100 column.The purity of PDJL-A11 was analyzed by UV spectrophotometer.The monosaccharide composition and molecular ratio of PDJL-A11 were analyzed by gas chromatography.The monosaccharide composition of PDJLA11 was composed of arabinose,fructose,mannose,rhamnose and glucose with a molar ratio of 2.11：6.52：8.81：12.59：15.58.

关 键 词：核桃隔膜 多糖 纯化 气相色谱 单糖组成 

分 类 号：TS201.23[轻工技术与工程—食品科学]