响应面分析法优化超声波提取密蒙花黄色素工艺  

Ultrasound-assisted Extraction of Yellow Pigment from Buddleja officinalis Maxim Flowers

作　　者：罗堾子[1,2] 孔永强 张弘[1] 郑华[1] 张加研[2] 李凯[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224 [2]西南林业大学木质科学与装饰工程学院,云南昆明650224 [3]景谷林化有限公司,云南景谷666400

出　　处：《食品科学》2010年第22期352-356,共5页Food Science

基　　金：科技部农业科技成果转化资金项目(2010GB24320619);国家林业科技成果推广项目([2010]11)

摘　　要：以水为介质、密蒙花黄色素水溶液的吸光度为考察指标,优化超声波强化提取密蒙花黄色素的工艺。选取超声波功率、提取时间和液料比为影响因素,在单因素试验基础上,进行三因素三水平的Box-Behnken中心组合试验设计,以密蒙花黄色素的吸光度为响应值进行响应面(RSM)分析,优化超声波强化提取密蒙花黄色素的提取条件。结果表明:超声波处理密蒙花有利于黄色素的浸出,超声波强化提取最佳条件为提取功率800W、提取时间24min、液料比27:1(mL/g);在最佳提取条件下,密蒙花黄色素吸光度理论值可达0.257,验证值为0.259,验证值与理论值间的相对误差为0.78%。密蒙花黄色素得率达5.95%。Yellow pigment in flowers of Buddleja officinalis Maxim was extracted by water with the assistance of ultrasound. The effects of ultrasound power, extraction time, water-to-solid ratio on the yield of yellow pigments （expressed as the absorbance of extract at 435 nm） were studied by conducting single factor tests. Then Box-Behnken experimental design involving the above 3 factors at 3 levels was employed, and coupled with response surface analysis to optimize the extraction conditions. Results showed that ultrasound treatment accelerated the extraction of yellow pigment. The optimum extraction conditions were as follows： ultrasound power 800 W, extraction time 24 min, the ratio of water to solid 27：1. Under such conditions, the theoretical absorbency of Buddleja officinalis Maxim yellow pigment can reach 0.257, while the experimental value is 0.259, with a relative stand error 0.78% to the theoretical value. The yield of yellow pigment reached up to 5.95% .

关 键 词：超声波提取 密蒙花黄色素 响应曲面 

分 类 号：TS202.3[轻工技术与工程—食品科学]