苯酚降解菌的固定化及其降解特性的研究  

Study on Immobilization of Phenol-Degrading Strain and Its Degradation Performance

作　　者：薛锋[1] 高健[1] 

机构地区：[1]盐城工学院化学与生物工程学院,江苏盐城224051

出　　处：《化学与生物工程》2010年第12期72-75,共4页Chemistry & Bioengineering

摘　　要：以海藻酸钠为载体,对苯酚降解菌进行细胞固定化,并对其降解特性进行了研究。通过单因素实验确定较佳的固定化条件为:海藻酸钠质量浓度3.0%、CaCl2质量浓度4.0%、湿菌体量0.4 g/10 mL海藻酸钠溶液;固定化细胞降解苯酚的最适条件为:温度30℃、pH值7.0、NaCl质量浓度低于2.5%,该菌株固定化细胞的降解苯酚能力和耐受苯酚能力均大于游离细胞,800 mg.L-1苯酚降解48 h,降解率可达99%以上。In this paper,the phenol-degrading strain was immobilized on sodium alginate.The optimal immobilization conditions were determined by single factor experiment as follows： sodium alginate of 3.0%,CaCl2 of 4.0%,amount of wet strain of 0.4 g.The optimum conditions for immobilized cell degrading phenol were as follows：temperature of 30℃,pH value of 7.0,NaCl mass concentration lower than 2.5%.The results showed that the degradation performance and tolerance of immobilized cells for phenol were apparently superoir to those of free cells.When 800 mg·L^-1 of phenol was degraded by immobilized cell for 48 h,its degradation rate reached over 99%.

关 键 词：苯酚 固定化 苯酚降解菌 海藻酸钠 

分 类 号：Q789[生物学—分子生物学] X172[环境科学与工程—环境科学]