栀子甾醇的分离分析  

Extraction and component analysis of phytosterol in Gardenia

作　　者：甘辉 万明 罗阳帆[3] 陈勇辉[3] 付桂明[3] 万茵[3] 

机构地区：[1]江西省南昌市东湖区卫生防疫站,330000 [2]南昌县莲塘镇农业服务中心,330200 [3]南昌大学生命科学与食品工程学院,330047

出　　处：《江西食品工业》2010年第4期35-37,共3页Jiangxi Food Industry

摘　　要：本文对栀子的植物甾醇成分进行分离分析,以期扩大栀子的应用范围。文中用索氏提取法从栀子中提取出果油,得率为16.7%。通过皂化法,用乙醚萃取出果油中的粗甾醇,再以溶剂结晶法对粗甾醇进行纯化。最后对所得甾醇样品的组分采用HPLC-MS分析。结果表明,栀子果油中可能含有β-谷甾醇和甘氨胆酸等甾醇成分。This paper was about the extraction and component analysis of phytosterol in Gardennia. The oil in the Gardennia was extracted by soxhlet extraction and the yield was 16.7%. Then the oil was saponified by ethanolic KOH, and the phytosterol in non-saponificable fraction was extracted with diethylether. Solvent crystallization was used to edulcorate phytosterol. Then the phytosterol was analyzed by HPLC-MS. The result showed that the component of Gardennia phytosterol maybe involved β-sitosterol and glycocholic acid.

关 键 词：栀子 植物甾醇 HPLC-MS 

分 类 号：TS207.3[轻工技术与工程—食品科学]