响应面法优化桃仁蛋白提取工艺  

Optimization of protein extraction from peach(Prunus persica L.) kernel using response surface methodology

作　　者：李静娟[1] 易建华[1] 朱振宝[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,西安710021

出　　处：《中国油脂》2010年第12期20-23,共4页China Oils and Fats

摘　　要：以脱脂桃仁粉为原料,研究了碱溶酸沉法提取桃仁蛋白的工艺条件。以桃仁蛋白提取率为指标,考察了提取温度、pH、料液比和提取时间对桃仁蛋白提取率的影响。在单因素实验基础上,通过响应面法(RSM)优化了提取工艺条件。结果表明,提取温度、pH和料液比对桃仁蛋白提取率影响较大;优化的桃仁蛋白提取工艺条件为:料液比1∶15,提取温度47℃,pH 9.2,提取时间1.0 h。在优化工艺条件下,桃仁蛋白的提取率达93.89%,其蛋白质含量为86.83%。Alkaline-extraction and acid-precipitation method was employed to prepare peach kernel protein from defatted peach kernel powder.The effects of extraction conditions including temperature,time,pH and solid-to-liquid ratio on the extraction rate of the protein were investigated.The extraction process was optimized by the response surface methodology（RSM）.The results showed that the temperature,pH and solid-to-liquid ratio had significant influences on the extraction rate.The optimum conditions were as follows： temperature 47℃,time 1.0 h,pH 9.2 and solid-to-liquid ratio 1∶15.Under the conditions the extraction rate of peach kernel protein was 93.89%,and the protein content reached 86.83%.

关 键 词：桃仁 桃仁蛋白 提取率 响应面 

分 类 号：TQ936.2[化学工程]