枯草芽孢杆菌B26生产多隔镰孢霉抑制物工艺优化  

Optimization of Medium Composition and Fermentation Conditions for the Production of Fusarium decemcellulare Brick Inhibitor by Bacillus subtilis B26

作　　者：张丽珍[1] 耿海峰[2] 樊晶晶[3] 牛伟 纪春[3] 赵婷婷[2] 牛宇[5] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西大学生物工程学院,山西太原030006 [3]山西大学生物技术研究所,山西太原030006 [4]山西省农业科学研究院,山西太原030006 [5]山西省农业科学院农业资源综合考察研究所,山西太原030006

出　　处：《食品科学》2010年第23期128-131,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD01A16);山西省留学人员科研项目(2008-119);山西省科技攻关项目(20100311001-7);山西省高校学科建设专项资金项目(2010)

摘　　要：采用正交试验设计对枯草芽孢杆菌B26抑制多隔镰孢霉培养基配方及用量进行优化,单因素试验对B26产生活性物质的发酵条件进行选择。以多隔镰孢霉的抑制率为指标,B26产生拮抗效果最好的发酵培养基配方用量是:蛋白胨25g/L、淀粉45g/L、氯化钠1.5g/L、硫酸铵1.0g/L、磷酸氢二钾1.5g/L。产生活性物质的最优发酵条件为:种子菌龄24h、接种量5%、初始pH7、装液量20%、吐温-80用量0.5%、发酵温度30℃、转速150r/min、发酵时间60h,获得B26菌株发酵液对多隔镰孢霉的抑制直径为26mm,优于其他条件培养获得的拮抗效果。A four-factor, three-level orthogonal array design was employed to optimize four medium components for the production of Fusarium decemcellulare Brick inhibitor by Bacillus subtilis B26, and the effects of fermentation conditions on the production of Fusarium decemcellulare Brick inhibitor were explored through single factor experiments. The optimal medium composition for maximizing inhibition rate against Fusarium decemcellulare Brick was found to be composed of peptone 25 g/L, starch 45 g/L, sodium chloride 1.5 g/L, ammonium sulfate 1.0 g/L, and dipotassium hydrogen phosphate 1.5 g/L. Seed age of 24 h, inoculmum size of 5%, initial pH of 7, the volume of medium in the fermentation vessel of 20%, the amount of added Tween -80 of 0.5%, fermentation temperature of 30 ℃, rotational speed of 150 r/min and fermentation period of 60 h were found optimal. The inhibition circle diameter of the fermentation broth obtained under these conditions was 26 mm.

关 键 词：多隔镰孢霉 生物防治 正交试验 发酵条件 

分 类 号：Q939.1[生物学—微生物学]