较长期喂养转人乳铁蛋白全乳粉对SD大鼠血清铁、铁蛋白含量的影响  

Effect of Transferred Human Lactoferrin Milk Powder on Serum iron and Ferritin in Rats

作　　者：祁潇哲[1] 王静[1] 周催[1] 许超[1] 车会莲[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2010年第23期340-343,共4页Food Science

基　　金：国家转基因生物新品种培育重大专项(2008ZX08011-006)

摘　　要：目的:饲喂SD大鼠转人乳铁蛋白全乳粉90d,监测其对大鼠营养状况以及生理、血液生化指标的影响,从而评价转人乳铁蛋白全乳粉在较长期喂养实验动物时对其血清铁、血清铁蛋白含量的影响。方法:参考中华人民共和国农业行业标准《转基因植物及其产品食用安全检测——大鼠90天喂养实验》(NY/T1102—2006)进行。将140只SD大鼠按体质量随机分为7组,每组20只,雌雄各半,分别为:常规饲料对照组,转基因奶粉低、中、高剂量组和非转基因奶粉低、中、高剂量组。90d后,观察各组实验动物体质量、食物利用率以及血生化指标。结果:转人乳铁蛋白全乳粉经过90d喂养实验动物后,各组动物生长发育良好,含转人乳铁蛋白全乳粉与不含转人乳铁蛋白全乳粉组相比,大鼠的体质量与食物利用率、总进食量均无显著差异(P≥0.05),在较长期喂养过程中未观察到含转人乳铁蛋白全乳粉对大鼠产生不良作用,含转人乳铁蛋白全乳粉可显著增加血清铁和铁蛋白水平(P≤0.05)。结论:SD大鼠较长期食用转人乳铁蛋白全乳粉未出现安全问题,且可以提高实验动物机体铁的营养状况。Objective： Feed on SD rats for 90 days with transferred human lactoferrin whole milk powder and their nutrition, physiological status, and biochemical indicators were investigated, thus to evaluate the influence of transferred human lactoferrin milk powder on rats serum iron and ferritin. Methods： The study refers to the People s Republic of China Agriculture Industry Standard Transgenic plants and products food safety testing （NY/T 1102—2006）. A total of 140 SD rats were divided into 7 groups, including control group, transgenic low-dose group, transgenic middle-dose group, transgenic high-dose group, non-transgenic low-dose group, non-transgenic middle-dose group, and non-transgenic high-dose group. Male and female were equally distributed. The weight, feed efficiency, and blood biochemical indicators during 90 days of feeding were measured. Results： SD rats fed on transferred human lactoferrin whole milk powder for 90 days were healthy, and compared with non-transgenic group, transgenic group did not show significant difference. Transgenic milk powder can increase the serum iron and ferritin in rats noticeably, and no adverse effects were observed during the study. Conclusion： Transferred human lactoferrin whole milk powder can increase the serum iron and ferritin in rats.

关 键 词：转人乳铁蛋白全乳粉 血清铁 血清铁蛋白 

分 类 号：R151.2[医药卫生—营养与食品卫生学] TS252.1[医药卫生—公共卫生与预防医学]