应用平板显色法测定单宁酶活力  

Determination of Tannase Activity by Color Plate Method

作　　者：聂光军[1,2] 刁金山[1] 刘会[1] 王丽[1] 郑之明[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,合肥230031 [2]安徽工程大学生物与化学工程学院,芜湖241000

出　　处：《应用与环境生物学报》2010年第6期883-887,共5页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院知识创新工程项目资助(No.KSCX2-YW-G-050)~~

摘　　要：为直观、批量、准确测定单宁酶的活力,以单宁酸为底物,溴甲酚绿为显色剂,采用平板显色法测定单宁酶的活力.对单宁酸浓度、溴甲酚绿浓度、琼脂浓度、酶加量以及反应时间的分析结果显示,平板组成为1%单宁酸、0.001%溴甲酚绿、3%琼脂,酶加量为30μL,40℃下反应24 h,透明圈清晰度最高,获得标准曲线的决定系数为98%,验证反应结果显示该方法准确、稳定.In order to make an audio-visual,batch and accurate assay of tannase activity,color plate method was applied with tannic acid and bromocresol green as substrate and chromogenic agent,respectively.The concentrations of tannic acid,bromocresol green and agar,addition of tannase and reaction time were studied and analyzed.The results showed that the 1% of tannic acid,0.001% bromocresol green,3% agar,30 μL tannase addition and 24 hours at 40 ℃ for reaction were the optimum for the plate method.The corresponding adjustable R square of standard curve was 98%.Verification experiment was carried out and its result shows that this method is accurate and stable for assaying tannase.

关 键 词：单宁酶 溴甲酚绿 单宁酸 琼脂 平板显色法 

分 类 号：Q946[生物学—植物学]