Proliferation inhibition of human cervical cancer HeLa cells by Casticin in vitro  

紫花牡荆素体外抑制人宫颈癌HeLa细胞增殖的研究(英文)

作　　者：Jing Xie Jun Bai Xifeng Sheng Jianguo Cao Wanyu Xie 

机构地区：[1]Department of Gynecology and Obstetrics, The First Affiliated Hospital of the University of South China, Hengyang 421001, China [2]Laboratory of Medicine Engineering, Medical College, Hunan Normal University, Changsha 410013, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2011年第1期47-50,共4页中德临床肿瘤学杂志（英文版）

基　　金：Supported by a grant from Hunan Provincial Health Department Research Fund (No.B2007-116)

摘　　要：Objective： The aim of the study was to investigate the effect of Casticin on proliferation inhibition of human cervical cancer HeLa cells in vitro and to unravel the associated mechanisms. Methods： Human cervical HeLa cells were cultured in vitro. The inhibitory effect of Casticin on the viability of human cervical cancer HeLa ceils was evaluated by the MTT assay. The colony formation ability was detected by plate colony formation assay. Distribution of cell cycle was analyzed by flow cytometry. The protein expression levels were analyzed by Western blot. Results： Casticin significantly inhibited the growth of human cervical cancer HeLa cells in a dose- and time-dependent manner, and the IC50 was 2.82 μg/mL. The colony-forming rate was reduced drastically compared with control group （P 〈 0.05）. The cells were markedly arrested at G2/M phase after the treatment of Casticin for 48 h. Western blot showed that the expression of p21 protein was up-regulated and protein level of Cyctin B1 was depressed by Casticin in a concentration dependent manner. Conclusion： Casticin could inhibit the cell growth and lead to cell arrest in human cervical cancer HeLa cells, and the down-regulation of Cyclin B1 protein expression and activation of p21 protein might contribute to Casticin induced cell arrest in human cervical cancer HeLa cells.

关 键 词：cervical cancer CASTICIN cyclin B1 P21 PROLIFERATION 

分 类 号：Q255[生物学—细胞生物学] Q813.11