大蒜素对人肝癌细胞HepG2的抑制和诱导细胞凋亡作用  

Allicin in vetro inhibits growth and induces apoptosis of human hepatoma HepG2 cells

作　　者：吕锋[1] 张园[2] 孙谦 孙志成[1] 谢朝良[4] 

机构地区：[1]扬州大学医学院附属兴化市人民医院感染病科,兴化市225700 [2]深圳市人民医院消化病科 [3]兴化市林湖乡卫生院 [4]泸州医学院二附院

出　　处：《江苏医药》2010年第23期2810-2812,共3页Jiangsu Medical Journal

摘　　要：目的观察大蒜素对人肝癌细胞HepG2的抑制和诱导细胞凋亡作用。方法用不同浓度大蒜素处理对数生长期的人肝癌细胞HepG2。比色还原法检查4、8、16 h时HepG2细胞生长的抑制率;Hoechest荧光染色观察8 h时细胞形态的变化;JC-1检测40 mg/L大蒜素作用4 h后细胞线粒体跨膜电位的变化;免疫组化法检测经浓度为40 mg/L大蒜素作用4 h后其对细胞色素C的影响。结果与无药阴性对照组比较,大蒜素能明显抑制HepG2细胞的增殖,且抑制率随浓度增加和作用时间延长而升高(P<0.01)。Hoechst 33258荧光染色观察到细胞凋亡形态学特征。结论 大蒜素对人肝癌细胞株HepG2有明显的生长抑制作用,诱导人肝癌细胞株HepG2的凋亡。这可能与其通过线粒体途径引起线粒体跨膜电位下降与细胞色素C的释放有关。Objective To study the effects of allicin in vetro inhibiting growth and inducing apoptosis of human hepatoma HepG2 cells.Methods HepG2 cells of log phase growth were incubated in vitro with allicin in different concentrations（20,40,60 mg/L） and for different times（4,8,16 h）.Cell proliferation was measured by MTT assay and the growth inhibition rate was calculated.Cell morphological changes at 8 h were observed with Hoechest fluorescent staining.Mitochondrial transmembrane potential treated with allicin 40 mg/L for 4 h was detected by mitochondrial apoptosis detection kit.Cytochrome C released from mitochondria was examined by immunohistochemistry method.Results Compared to negative control,the proliferation of HepG2 cells was inhibited by allicin in the concentration-and time-dependent manner（P0.01）.The apoptosis of HepG2 cells treated with allicin was seen.Conclusion Allicin has significant effects of inhibiting growth and inducing appoptosis of HepG2 cells,which may be related to increased release of cytochrome C from mitochondria and decreased mitochondrial transmembrane potential.

关 键 词：大蒜素 HEPG2细胞 凋亡 细胞色素C 

分 类 号：R735[医药卫生—肿瘤]