金属络合法纯化银杏黄酮的研究  

Purification of Ginkgo Flavonoid by Metal Complex Method

作　　者：张静[1] 张晓鸣[1] 佟建明[1,2] 夏书芹[1] 

机构地区：[1]食品科学与技术国家重点实验室江南大学食品学院,无锡214122 [2]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《天然产物研究与开发》2010年第5期751-754,776,共5页Natural Product Research and Development

基　　金：国家"十一五"科技支撑项目(2006BAD12B05)

摘　　要：为寻找一种生产周期短、分离效果好、易操作的纯化黄酮的方法,本研究利用黄酮与金属能形成络合物的性质,从反应体系溶剂、金属盐、解络合剂的添加量优化了络合法纯化银杏黄酮的工艺。结果表明,pH值对络合反应的发生及最终产品的性质具有很重要的影响。金属络合纯化法的最佳工艺条件为:以100 mL甲醇为溶剂,黄酮浓度为2.4 mg/mL,0.20 g硫酸锌为络合剂,反应溶液pH值为9.50,0.2 g EDTA为解络合剂,可以使银杏提取物中黄酮含量由24%提高到55%,得率为58%。To develop a flavonoid-complex method for purification of flavonoid,the purification conditions for ginkgo flavonoid were studied which included the solvent,the metal,the pH value for complex formation,and the EDTA for decomposition the complex.The results indicated that the pH value was the most important factor to the purification process and the optimized conditions to purify ginkgo flavonoids were as follow：100 mL methanol as solvent,the ginkgo flavonoid concentration was 2.4 mg/ml,0.20 g zinc sulfate as chelating agent,pH value at 9.50,and EDTA（0.20 g） as reagent to decompose complex.After one turn,the flavonoid content was increased from 24% to 55% and the recovery was 58%.

关 键 词：银杏黄酮 金属 PH值 络合 纯化 

分 类 号：TS202.3[轻工技术与工程—食品科学]