马铃薯渣高效降解菌的紫外诱变选育及发酵试验  

UV mutagenesis in efficient degradation strains of potato pulp and fermentation

作　　者：马振贵[1] 马伟超[1] 蒲建平[1] 安建平[1] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070

出　　处：《中国酿造》2011年第1期123-126,共4页China Brewing

基　　金：甘肃省科技厅资助项目(090NKCE081);天水师范学院中青年教师科研资助项目(TSA0918)

摘　　要：以选育马铃薯渣高效降解菌为目的，对康宁木霉进行紫外诱变，以致死率在80％-90％之间的菌株进行初筛，通过羧甲基纤维素钠刚果红筛选平板复筛，选育出两株具有较高酶活的菌株，并命名为P12、P13。通过诱变，完全培养基中P12的纤维素酶活（CMCA）及滤纸酶活（FPA）分别提高了1．45倍、1．738倍，P13的羧甲基纤维素酶活（CMCA）及滤纸酶活（FPA）分别提高了1．4倍和1．667倍。并通过发酵试验，发酵培养基CMC酶活分别是初始菌株的1．72倍和1．84倍。P12和P13具有良好的降解马铃薯渣的能力。Trichoderma koningii is mutated by UV for the selection of efficient degradation strains of potato pulp. The treated strains with a death rate of 80%~90% were preliminarily screened, and then were secondly screened by CMC Congo red agar plate, by which two strains with higher activity on degradation were isolated and named as P12 and P13. After mutation, the CMC activity （CMCA） and filter paper activity （FPA） of cellulase in P12 were improved 1.45 times and 1.738 times respectively in full media, while the CMCA and FPA in P13 were improved 1.4 times and 1.667 times respectively. In fermentation, the CMC activities of P 12 and P 13 were 1.72 times and 1.84 times higher than that of the initial strain, respectively. It was indicated that P12 and P13 had good abilities on the degradation of potato pulp.

关 键 词：康宁木霉 紫外诱变 酶活 发酵试验 

分 类 号：Q933[生物学—微生物学]