多孔介质渗透率对油藏微生物生长代谢影响  

Effects of permeability of porous medium on the growth and metabolism of microorganism in reservoir

作　　者：包木太[1,2] 袁书文[1,2] 李希明[3] 宋智勇[3] 郭辽源[3] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,青岛266100 [2]中国海洋大学化学化工学院,青岛266100 [3]中国石化胜利油田分公司采油工艺研究院,山东东营257000

出　　处：《深圳大学学报（理工版）》2011年第1期35-40,共6页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(50604013)~~

摘　　要：采油微生物在提高原油采收率过程中,受油藏环境条件,包括温度、压力、矿化度、pH值和渗透率等因素影响.用人工填沙岩心模拟地层多孔介质,以淀粉水解液为主要碳源,通过对胜利油田中一区Ng3产出水中的内源微生物,在60℃高温和10MPa高压下的静态激活实验,考察在不同渗透率的多孔介质中,采油微生物的激活代谢情况.利用聚合酶链反应-变性梯度凝胶电泳(polymerase chain reaction-denaturing gradientgel electrophoresis,PCR-DGGE)技术,研究不同渗透率岩心激活后微生物群落DGGE条带的变化.结果表明,微生物在不同渗透率多孔介质中的生长代谢有较大差异,岩心渗透率显著影响微生物群落结构,高渗透多孔介质培养条件下微生物种类比低渗透介质条件下多,丰度也较高.There are many environmental factors,such as temperature,pressure,salinity,pH,and permeability, affecting microbial enhanced oil recovery （MEOR）.The microbial metabolism in various porous media permeability under high temperature （60 ℃） and high pressure （10 MPa） was investigated.The strata in porous media was simulated by sand packed column.The in-situ microorganism of produced water from Zhongyi block in Shengli Oil Field was cultured in the sand packed column with starch hydrolysates,which served as the major carbon source.The changes of microbial community in different permeability activation were explored by PCR-DGGE technique.The results showed that the growth and metabolism of microorganism varied with different permeability of porous media,and the core permeability significantly affected the microbial community structure.The species and abundance of microorganisms in high permeability porous media were more than those in low permeability porous media.

关 键 词：采油工程 环境微生物 油藏 渗透率 内源微生物采油 聚合酶链反应-变性梯度凝胶电泳 

分 类 号：X172[环境科学与工程—环境科学] TE357.9[石油与天然气工程—油气田开发工程]