产蛋白酶和纤维素酶纳豆芽孢杆菌(B. Subtilis Natto)的筛选及其生物特性研究  

Screening of Protease and Cellulose Producing Bacillus Subtilis Natto Strains and their Biological Characteristics

作　　者：奚晓琦[1,2] 王加启[2] 卜登攀[2] 孙妍[2] 周凌云[2] 魏宏阳[2] 

机构地区：[1]东北农业大学动物科学与技术学院,黑龙江哈尔滨150030 [2]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193

出　　处：《中国畜牧兽医》2011年第1期33-38,共6页China Animal Husbandry & Veterinary Medicine

基　　金：“十一五”国家奶业科技支撑计划(2006BAD12B03);动物营养学国家重点实验室(2004DA125184(团)0801)

摘　　要：采用酪蛋白平板和羧甲基纤维素钠（CMC-Na）平板初筛法,分别从北京、大连、日本3个产地的纳豆食品中分离筛选到10株产蛋白酶菌株（NY-1-NY-10）和10株产纤维素酶菌株（NS-1-NS-10）。经酶活力测定NY-1为蛋白酶活力最高菌株,NS-1为纤维素酶活力最高菌株。通过2株菌的生长曲线、芽孢形成曲线、产酶动态变化曲线和酶活力传代稳定性试验,得出NY-1的最佳种龄为16 h,最佳芽孢收获时间为28 h,芽孢量的对数值为（6.92±0.047）、蛋白酶产酶高峰时间为18 h,酶活力为（44.12±1.48）U/mL,;NS-1的最佳种龄为14 h,最佳芽孢收获时间为28 h,芽孢量的对数值为（8.41±0.0060）,纤维素酶产酶高峰时间为40 h,酶活力为（60.94±1.22）U/mL,蛋白酶和纤维素酶酶活力在7代内保持稳定。Using casein plate and carboxymethylcellulose sodium（CMC-Na） plate screening method,ten protease activity producing Bacillus Subtilis Natto strains（NY-1 to NY-10） and ten cellulose producing Bacillus Subtilis Natto strains（NS-1 to NS-10） were screened from Beijing,Dalian and Japanese Natto.NY-1 and NS-1 were selected from the examined strains for their highest protease activity and highest cellulose activity,respectively.NY-1 and NS-1 were studied with respect to their biological characteristics,including growth curves,sporulation curve,dynamic changes of enzyme activity and passage stability.The experimental results demonstrated that for strain NY-1 the best culture time was 16 h,the maximum logarithm of spores were（6.92±0.047） at 28 h,the maximum protease activity was（44.12±1.48） U/mL at 18 h;for strain NS-1 the best culture time was 14 h,the maximum logarithm of spores was（8.41±0.0060） at 28 h,the maximum cellulose activity was（60.94±1.22） U/mL at 40 h.Protease activity of NY-1 and cellulose activity of NS-1 will keep a good passage stability within seven generations.

关 键 词：纳豆芽孢杆菌 生长曲线 芽孢 蛋白酶 纤维素酶 筛选 传代 

分 类 号：S816.79[农业科学—饲料科学]