正交实验法优选绿茶绿色素的超声波提取工艺  

Optimum extraction of green pigment from green tea by ultrasonic-wave and orthogonal test

作　　者：张吉祥[1] 

机构地区：[1]廊坊师范学院化材学院,廊坊065000

出　　处：《食品科技》2011年第1期192-196,共5页Food Science and Technology

基　　金：河北省教育厅基金项目(2007317);廊坊师范学院科学基金项目(LSZB200801)

摘　　要：采用正交实验法,利用超声提取技术,优选由绿茶中提取绿色素的最佳工艺条件。以绿色素的乙醇浸提液在可见光区665nm处的吸光度为考察指标,应用正交实验法对提取过程中浸提温度(A)、浸提时间(B)、超声波功率(C)、乙醇浓度(D)及料液比(E)5个因素进行优选研究。结果表明,乙醇浓度与浸提温度对茶叶中绿色素提取率的影响较为显著,最佳工艺条件为乙醇浓度85%、浸提温度60℃、料液比1:16、提取时间40min、超声波功率350W。在此优化条件下绿茶中绿色素提取率达到38.80%,为先前文献报导的1.7倍。The optimum technology for ultrasonic-assisted extraction of green pigment from green tea was investigated in this paper.The absorbency of green pigment solution at the wavelength 665 nm as research index of the extraction yield was measured by spectrometry.The orthogonal test was conducted to evaluate the effects of five factors the extraction temperature（A）,extraction time（B）,ultrasonic power（C）,ethanol concentration（D）,material/solvent ratio（E） on the extraction yield of Tea Creen pigment（TGP）.The results showed that the ethanol concentration and extraction temperature have significant effects on the yield of TGP.The optimal parameters of the extraction process were as follows： ethanol concentration（85%）,extraction temperature（60 ℃）,material/solvent ratio（1：16）,extraction time（40 min）,ultrasonic power（350 W）.Under the optimum experimental condition,the extraction yield of green pigment from green tea reached at 38.80%,which is 1.7 times the yield that reported in former paper.

关 键 词：正交实验法 绿茶 绿色素 超声波 提取 

分 类 号：TS202.3[轻工技术与工程—食品科学]