羊水细胞培养及染色体核型分析在产前诊断中的应用  

The Value of Culturing Amniotic Fluid Cell and Chromosome Analysis in Prenatal Diagnosis

作　　者：胡美红[1] 刘雨生[1] 何国平[1] 陈玲[2] 周桂香[1] 宋雅娴[1] 童先宏[1] 骆丽华[1] 

机构地区：[1]安徽医科大学附属省立医院生殖医学中心,合肥230001 [2]安徽医科大学附属省立医院妇产科

出　　处：《临床输血与检验》2011年第1期23-25,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探讨羊水细胞培养及染色体核型分析在产前诊断中的应用价值。方法从中孕期妇女羊膜腔穿刺获取羊水,细胞培养后分析染色体核型,进行产前诊断。结果 17例产前诊断患者羊水细胞培养及制片成功率均为100%,共检出异常染色体核型3例,检出率为17.65%,其中21三体2例(11.76%),胎儿2N/4N嵌合体1例(5.88%)。结论羊水细胞培养及染色体核型分析是目前安全、有效、可靠的产前诊断方法之一,结合唐氏筛查、超声诊断、脐血染色体检查可有效预防出生缺陷的发生。Objective To evaluate the feasibility and reliability of using cultured amniotic fluid cells and chromosome analysis for prenatal diagnosis.Methods Amniotic fluid cells were cultured for chromosome analysis.Results 3 cases of chromosome abnormalities were detected in 17 cases of amniotic fluid cells and the detection rate of chromosome abnormalities was 17.65%.Among the chromosome abnormalities 2 cases were Trisomy 21（11.76%） and the other cases were chromosome mosaicism 2N/4N（5.88%）.Conclusion Culturing amniotic fluid cells and chromosome analysis are safe,effective and reliable methods for prenatal diagnosis of high-risk pregnant women,which play an important role in guiding prenatal and postnatal care and improving population quality.

关 键 词：产前诊断 羊水细胞 染色体核型分析 唐氏综合征 

分 类 号：R714.55[医药卫生—妇产科学]