Comparative proteome analysis of alkaliphilic Bacillus sp.N16-5 grown on different carbon sources  

作　　者：LI Gang SONG YaJian XUE YanFen RAO Lang ZHOU Chen WANG QuanHui MA YanHe 

机构地区：[1]State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China [3]Beijing Proteomics Institute, Beijing 101318, China

出　　处：《Science China(Life Sciences)》2011年第1期90-100,共11页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China, Ministry of Science and Technology of China (Grant Nos. 2007CB707801 and 2003CB716001);the National High Technology Research and Development Program of China (Grant Nos. 2006AA020201 and 2007AA021306)

摘　　要：To determine the impact of carbohydrates on the metabolic pathway in alkaliphiles, proteomes were obtained from cultures containing different carbohydrates and were resolved on two-dimensional gel electrophoresis (2-DE). The proteomes were compared to determine differentially expressed proteins. A novel alkaliphilic bacterium (alkaliphilic Bacillus sp. N16-5 isolated from Wudunur Soda Lake, China) was isolated in media with five different carbon sources (glucose, mannose, galactose, arabinose, and xylose). Comparative proteome analysis identified 61 differentially expressed proteins, which were mainly involved in carbohydrate metabolism, amino acid transport, and metabolism, as well as energy production and conversion. The comparison was based on the draft genome sequence of strain N16-5. The abundance of enzymes involved in central metabolism was significantly changed when exposed to various carbohydrates. Notably, catabolite control protein A (CcpA) was up-regulated under all carbon sources compared with glucose. In addition, pentose exhibited a stronger effect than hexose in CcpA-mediated carbon catabolite repression. These results provided a fundamental understanding of carbohydrate metabolism in alkaliphiles.To determine the impact of carbohydrates on the metabolic pathway in alkaliphiles, proteomes were obtained from cultures containing different carbohydrates and were resolved on two-dimensional gel electrophoresis （2-DE）. The proteomes were compared to determine differentially expressed proteins. A novel alkaliphilic bacterium （alkaliphilic Bacillus sp. N16-5 isolated from Wudunur Soda Lake, China） was isolated in media with five different carbon sources （glucose, mannose, galactose, arabinose, and xylose）. Comparative proteome analysis identified 61 differentially expressed proteins, which were mainly involved in carbohydrate metabolism, amino acid transport, and metabolism, as well as energy production and conversion. The comparison was based on the draft genome sequence of strain N16-5. The abundance of enzymes involved in central metabolism was significantly changed when exposed to various carbohydrates. Notably, catabolite control protein A （CcpA） was up-regulated under all carbon sources compared with glucose. In addition, pentose exhibited a stronger effect than hexose in CcpA-mediated carbon catabolite repression. These results provided a fundamental understanding of carbohydrate metabolism in alkaliphiles.

关 键 词：Bacillus sp. N16-5 ALKALIPHILE cytoplasmic protein 2-DE 

分 类 号：TQ925[轻工技术与工程—发酵工程] S512.101[农业科学—作物学]