Arthrobacter sp.No.3细菌酶转化三醇类人参皂苷Rg_1生成Rh_1的反应条件  

Transformation of ginsenoside Rg_1 to ginsenoside Rh_1 by the enzyme from Arthrobacter sp.No.3 bacterium

作　　者：孙斯宜[1] 宋建国[2] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连工业大学化工与材料学院,辽宁大连116034

出　　处：《大连工业大学学报》2011年第1期1-4,共4页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省教育厅创新团队项目(LG2010009)

摘　　要：为了提高Arthrobactersp.No.3 GS 0202新细菌所产人参皂苷糖苷酶水解人参三醇类皂苷Rg1生成人参三醇类皂苷Rh1的能力,采用薄层层析法和高效液相色谱法,对该菌的发酵条件及所产糖苷酶的反应条件进行了研究。结果表明,在30℃发酵条件下,人参皂苷酶的诱导物人参茎叶总皂苷存在下,该细菌产生较好。其粗酶的最佳反应条件为人参皂苷Rg1质量浓度0.1 mg/mL,30℃下恒温反应24 h。该菌株所产酶液与人参三醇类皂苷Rg1反应后,检测发现该酶液可将人参三醇类皂苷Rg1转化为人参三醇类皂苷Rh1。高效液相色谱法检测,该酶在最佳反应条件下,能够将20%的Rg1转化为Rh1。In this paper,the condition for ginsenosidase production and reaction from Arthrobacter sp.No.3 GS0202 strain was examined by TLC and HPLC.The ginsenosidase was produced in the medium containing 1% triptone,0.5% yeast extract and 1% NaCl at 30 ℃,using total ginsenoside as inducer.The optimal conditions for hydrolysis of ginsenoside Rg1 were at 30 ℃ for 24 h and the concentration of substrate was 0.1 mg/mL.The enzyme is able to hydrolyze the 20-O-b-D-glucoside of the protopanaxatriol ginsenoside Rg1 to the ginsenoside Rh1,and the yield of ginsenoside Rh1 was approximately 20% detected by HPLC.

关 键 词：人参三醇类皂苷Rh1、Rg1 酶水解 薄层层析 高效液相色谱 

分 类 号：TS201.25[轻工技术与工程—食品科学] Q555.4[轻工技术与工程—食品科学与工程]