G8r菌产人参皂苷糖苷酶的酶性质及酶水解作用  

Characterization of ginsenoside-glyosidase from Absidia sp.G8r

作　　者：陈娇娇[1] 孙键[1] 金凤燮[1] 鱼红闪[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2011年第1期13-17,共5页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省科学技术基金资助项目(20042132);辽宁省教育厅创新团队项目(2009T007)

摘　　要：对由Absidiasp.G8r菌产的人参皂苷糖苷酶(Glc8)进行了分离纯化,并对其酶性质和酶水解作用进行了研究。结果表明,该酶的分子质量约为72 ku,酶反应的最适温度和pH分别为30℃和5.0,在20～60℃、pH3.0～7.0条件下稳定性较好。该酶水解人参皂苷Rb1生成人参皂苷C-K,水解过程是分步进行的,酶先水解Rb1生成Rd;大约8 h,酶水解Rd生成F2;大约18 h,酶水解F2生成C-K。并对此过程的酶反应动力学进行研究,米氏常数分别为5.488、5.176、5.495 mmol/L,最大反应速率分别1.524、0.904、0.447 mmol/(L.h)。The ginsenoside-glyosidase produced by Absidia sp.G8r was purified and characterized.The result indicated that its molecular weight is about 72 ku and the optimal temperature and pH for enzyme reaction were 30 and 5.0 respectively Enzyme is stable at 20-60 ℃,pH 3.0-7.0.Ions K＋,Na＋,Ca2＋,Mg2＋,Zn2＋ had no effect on the enzyme activity,while Cu2＋ inhibited the enzyme activity.The enzyme hydrolyze Rb1 to C-K,in which Rb1 was hydrolyzed to Rd first in about 8 h,then Rd was hydrolyzed to F2 in about 18 h,and after that F2 was hydrolyzed to C-K.The Km of the enzyme are 5.488,5.176,5.495 mmol/L,the Vmax of the enzyme are 1.524,0.904,0.447 mmol/（L·h）.

关 键 词：人参皂苷 人参皂苷糖苷酶 酶性质 水解作用 

分 类 号：TS201.25[轻工技术与工程—食品科学] Q556.2[轻工技术与工程—食品科学与工程]