MicroRNA-17-5p反义寡核苷酸阻滞白血病K562细胞的细胞周期  

MicroRNA-17-5p specific antisense oligonucleotide inhibits cell cycle of leukemia K562 cells

作　　者：马卓娅[1] 李欣[2] 汤华[2] 刘民[2] 

机构地区：[1]深圳市儿童医院儿科疾病研究所,广东深圳518026 [2]天津医科大学生命科学中心,天津300070

出　　处：《中国肿瘤生物治疗杂志》2010年第6期644-647,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.30873017)~~

摘　　要：目的:以反义寡核苷酸技术研究microRNA-17-5p(miR-17-5p)对人慢性髓系白血病K562细胞增殖的影响及其可能的机制。方法:脂质体法将miR-17-5p反义寡核苷酸(miR-17-5p antisense oligonucleotide,miR-17-5p-ASO)和对照无义寡核苷酸(control nonsense oligonucleotide,Ctrl-NSO)转染入K562细胞,同时设未转染对照组(Ctrl)。MTT法检测K562细胞的增殖,TUNEL法检测K562细胞的凋亡,流式细胞术检测K562细胞周期的改变。结果:MTT检测结果显示,miR-17-5p-ASO组K562细胞增殖活性为Ctrl-NSO组的(61.7±4.7)%,miR-17-5p-ASO转染显著抑制K562细胞的增殖(P<0.05)。TUNEL检测显示,miR-17-5p-ASO转染不影响K562细胞凋亡(P>0.05);miR-17-5p-ASO组K562细胞G2期细胞比例为(10.8±0.8)%,显著低于Ctrl-NSO组和Ctrl组的(34.6±0.4)%和(33.9±1.3)%(P<0.05)。结论:MiR-17-5p反义寡核苷酸可以通过调控细胞周期抑制K562细胞的增殖,有望成为白血病治疗的新手段。Objective：To study the effect of microRNA-17-5p （miR-17-5p） on the growth of chronic myelocytic leukemia K562 cells by antisense oligonucleocide technique.Methods：miR-17-5p antisense oligonucleotide （miR-17-5p-ASO） and control nonsense oligonucleotide （Ctrl-NSO） were transfected into K562 cells by Lipofectamine assay,and un-transfected K562 cells were used as blank group （Ctrl）.The proliferation,apoptosis,and cell cycle changes of K562 cells were examined by MTT,TUNEL,and flow cytometry assays,respectively.Results： MTT results showed that the proliferation of K562 cells in miR-17-5p-ASO group was （61.7±4.7）% of that in Ctrl-NSO group,and miR-17-5p-ASO transfection significantly inhibited the proliferation of K562 cells （P0.05）.TUNEL results showed that miR-17-5p-ASO transfection did not affect apoptosis of K562 cells （P0.05）.The ratio of K562 cells in G2 phase was （10.8±0.8）% in miR-17-5p-ASO group,which was significantly lower that those in Ctrl-NSO and Ctrl groups （[34.6±0.4]%,[33.9±1.3]%,all P0.05）.Conclusion： MiR-17-5p specific antisense oligonucleotide can suppress K562 cells growth by inhibiting cell cycle,which may provide a new way for treatment of leukemia.

关 键 词：microRNA-17-5p 反义寡核苷酸 白血病 K562细胞 细胞周期 

分 类 号：R733.7[医药卫生—肿瘤] R730.54[医药卫生—临床医学]