瘤胃菌与丁醇菌共生发酵麸皮生产丁醇  

Butanol production by rumen bacterium and C. acetobutylicum symbiosis fermentation with wheat bran

作　　者：应明[1] 黄贵明[2] 

机构地区：[1]天津理工大学化学化工学院,天津300384 [2]天津市新天进科技开发有限公司,天津300193

出　　处：《化学工程》2010年第12期72-75,共4页Chemical Engineering(China)

基　　金：天津市滨海新区双百科技特派员科技专项(SB20080078)

摘　　要：寻求瘤胃纤维素降解菌群NLH151与丁醇菌zr11共生发酵麸皮生产丁醇的最佳工艺条件。考察了接种方式、共培养温度、pH值等因素对纤维素降解率及丁醇生产能力的影响,得出了2种菌最佳共生培养的发酵条件。共生发酵时,麸皮质量浓度为20 g/L,共培养温度为33℃,pH值为6.0—6.5,瘤胃菌NLH151与丁醇菌zr11的接种量(体积分数)分别控制在15%和8%,瘤胃菌接入24 h后接入丁醇菌,共发酵48 h,总溶剂质量浓度可达到10.33 g/L,其中丁醇为7.8 g/L。该研究为利用农业废弃物进行新能源的开发提供了实验依据。The optimum conditions of producing butanol by rumen bacterium NLH151 and C.acetobutylicum zr11 symbiosis fermentation with wheat bran were researched.The effects of inoculation form,temperature,pH value,et al.on bran cellulose degradation and fermentation were studied.The optimum fermentation conditions were obtained as follows： wheat bran mass concentration 20 g/L,temperature 33 ℃,pH value 6.0-6.5,inoculation quantities（volume fraction） of NLH151 and C.acetobutylicum zr11 15% and 8% respectively.C.acetobutylicum zr11 was inoculated and symbiotically fermented for 48 h after 24 h NLH151 inoculation.The mass concentration of total solvent is 10.33 g/L,in which butanol mass concentration is 7.8 g/L.This result provides the basic data for the development of new energy with corn solid waste.

关 键 词：麸皮 瘤胃菌 C.acetobutylicum 丁醇发酵 共生发酵 

分 类 号：TQ223.124[化学工程—有机化工]