Taurolidine联合X射线对小鼠恶性黑色素瘤细胞周期进程的影响  

Effects of taurolidine in combination with X-rays irradiation on cell cycle progression of murine melanoma cells

作　　者：孙宝胜[1,2] 张矛[1] 刘林林[3] 张伟静[3] 孙娟[4] 龚守良[2] 

机构地区：[1]吉林省肿瘤医院放疗三科,吉林长春130012 [2]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [3]吉林大学第二医院肿瘤生物治疗科,吉林长春130041 [4]吉林省前卫医院内科,吉林长春130012

出　　处：《吉林大学学报（医学版）》2011年第1期21-25,共5页Journal of Jilin University：Medicine Edition

基　　金：爱尔兰国立科技发展基金资助课题(2009-2010)

摘　　要：目的:观察taurolidine联合X射线照射对小鼠恶性黑色素瘤(B16-4A5和B16-F10)细胞周期进程的影响,探讨其诱导肿瘤细胞凋亡的发生机制。方法:选择B16-4A5和B16-F10细胞系按给药浓度随机分为4组,taurolidine剂量分别为0、25、50和100μmol.L-1,同时进行1、2和4Gy X射线照射,采用流式细胞术检测细胞凋亡率和细胞周期,Western blotting分析cyclin B、cdc2和caspase-3的表达。结果:与25μmol.L-1taurolidine组比较,50和100μmol.L-1 taurolidine组诱导B16-4A5和B16-F10细胞发生G0/G1期阻滞,细胞数分别升高54.9%、73.7%和36.8%、55.5%(P<0.05);50μmol.L-1 taurolidine联合2和4Gy X射线照射组,细胞G2/M期阻滞消除,细胞数分别降低52.1%、44.2%和59.3%、52.7%(P<0.05)。与对照组、单纯taurolidine组及单纯照射组比较,联合4Gy X射线照射组cyclin B和cdc2的表达降低,caspase-3的表达升高(P<0.05)。结论:taurolidine联合X射线照射可去除G2/M期阻滞,可选择地抑制肿瘤细胞cyclin B和cdc2的表达、增强caspase-3的表达,共同诱导细胞凋亡。Objective To evaluate the effects and mechanisms of taurolidine in combination with X-rays irradiation on cell cycle progress of murine melanoma cells,and provide a new idea of malignant tumor clinical treatment.Methods B16-4A and B16-F10 cells were divided into 4 groups according to the concentrations of taurolidine（0,25,50 and 100 μmol·L-1）,respectively and combined with 1,2 and 4 Gy X-rays irradiation.The apoptotic rate and cell cycles were detected by flow cytometry.The expressions of cyclinB,cdc2,and caspase-3 were detected by Western blotting.Results Compared with 25 μmol·L-1 taurolidine group,the arrest of G2/M phase of B16-4A and B16-F10 cells after treated with 50 and 100 μmol·L-1 taurolidine was found and the cell numbers were increased by 54.9%,73.7% and 36.8%,55.5%,respectively（P0.05）.The arrest of G2/M phase of B16-4A and B16-F10 cells was also found and the cell numbers were decreased by 52.1%,44.2% and 59.3%,52.7%（P0.05）,respectively,after treated with 50 μmol·L-1 taurolidine in combination with 2 and 4 Gy X-rays irradiation.Therefore,the increase of caspase 3 expression and the decrease of cyclin B and cdc2 expressions were induced by the combination of 50 μmol·L-1 taurolidine with 2 and 4 Gy X-rays irradiation compared with control,taurolidine alone and irradiation alone groups.Conclusion Taurolidine in combination with X-rays irradiation can delete the G2/M arrest,supress the expressions of cyclin B and cdc2,increase the expression of caspase-3 and induce the apoptosis.

关 键 词：TAUROLIDINE X射线 细胞周期 细胞分裂周期2 细胞周期蛋白B 

分 类 号：R730.44[医药卫生—肿瘤]