Effects of Autoclaving and Mercuric Chloride Sterilization on PAHs Dissipation in a Two-Liquid-Phase Soil Slurry  

作　　者：WANG Cong-Ying WANG Fang WANG Tao YANG Xing-Lun BIAN Yong-Rong F. O. KENGARA LI Zeng-Bo JIANG Xin 

机构地区：[1]State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China) [2]School of Environmental Science and Resources, Shanxi University, Taiyuan 030006 (China) [3]Department of Chemistry, Maseno University, Private Bag Maseno (Kenya) [4]Institute of Chemical and Biological Technology, Taiyuan University of Science and Technology, Taiyuan 030021 (China)

出　　处：《Pedosphere》2011年第1期56-64,共9页土壤圈（英文版）

基　　金：Supported by the National High Technology Research and Development Program (863 Program) of China (No. 2007AA061101);the National Natural Science Foundation of China (Nos. 20707028,4092106,40771104 and 40701078)

摘　　要：A two-liquid-phase （TLP） soil slurry system was employed to quantify the efficiencies of autoclaving and mercuric chloride sterilization in the dissipation of polycyclic aromatic hydrocaxbons （PAHs）. The fates of 11 PAHs （naphthalene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo（a）anthracene, benzo（a）pyrene, benzo（b）fluoranthene, benzo（k）fluoranthene, dibenzo（a, h）anthracene） were recorded over 113 days of incubation. No microorganisms were detected in the HgC12-sterilized soil slurries during the whole incubation period, indicating very effective sterilization. However, about 2% 36% losses of PAHs were observed in the HgCl2- sterilized slurry. In contrast to the HgCl2-sterilized soil slurry, some microorganisms survived in the autoclaved soil slurries. Moreover, significant biodegradiation of 6 PAHs （naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene） was observed in the autoclaved soil slurries. This indicated that biodegradation results of PAHs in the soil slurries, calculated on basis of the autoclaved control, would be underestimated. It could be concluded that the sterilization efficiency and effectiveness of HgCl2 on soil slurry was much higher than those of autoclaving at 121 ℃ for 45 rain.A two-liquid-phase(TLP) soil slurry system was employed to quantify the efficiencies of autoclaving and mercuric chloride sterilization in the dissipation of polycyclic aromatic hydrocarbons(PAHs).The fates of 11 PAHs(naphthalene,fluorene,phenanthrene,anthracene,fluoranthene,pyrene,benzo(a)anthracene,benzo(a)pyrene,benzo(b)fluoranthene,benzo(k)fluoranthene,dibenzo(a,h)anthracene) were recorded over 113 days of incubation.No microorganisms were detected in the HgCl 2-sterilized soil slurries during the whole incubation period,indicating very effective sterilization.However,about 2%-36% losses of PAHs were observed in the HgCl 2 sterilized slurry.In contrast to the HgCl 2-sterilized soil slurry,some microorganisms survived in the autoclaved soil slurries.Moreover,significant biodegradation of 6 PAHs(naphthalene,fluorene,phenanthrene,anthracene,fluoranthene and pyrene) was observed in the autoclaved soil slurries.This indicated that biodegradation results of PAHs in the soil slurries,calculated on basis of the autoclaved control,would be underestimated.It could be concluded that the sterilization efficiency and effectiveness of HgCl 2 on soil slurry was much higher than those of autoclaving at 121℃ for 45 min.

关 键 词：adsorption aqueous solubility BIODEGRADATION MICROORGANISMS octanol-water partition coefficient 

分 类 号：X132[环境科学与工程—环境科学] TS203[轻工技术与工程—食品科学]