成肌细胞与雪旺细胞混合移植治疗肌营养不良的初步研究  

Co-transplantation of Myoblasts and Schwann Cells in the Therapy of Duchenne Muscular Dystrophy

作　　者：罗丽[1,2] 周红雨[1] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]成都三六三医院神经外科,成都610041

出　　处：《四川大学学报（医学版）》2011年第1期101-105,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30200085)资助

摘　　要：目的观察成肌细胞与雪旺细胞局部混合移植治疗Duchenne型肌营养不良症(DMD)模型鼠(mdx鼠)后抗肌萎缩蛋白(dys)的表达及成肌细胞与宿主的融合情况。方法成肌细胞和雪旺细胞培养、纯化、鉴定后,将其按比例(10:1)混合注射到mdx鼠的左侧腓肠肌内(混合移植组),同时设单纯移植组(仅注射成肌细胞),两组右侧腓肠肌均注射DF培养基作为对照。细胞移植后1月取腓肠肌进行免疫荧光染色,观察骨骼肌dys表达情况。结果混合移植组左侧腓肠肌dys的积分光密度(IOD)值(6342429.4167±925414.20068)高于单纯移植组(5295615.7500±1243283.86373,P<0.05),而且两组左侧腓肠肌dys的IOD值均比同组右侧腓肠肌高(P<0.05)。结论成肌细胞雪旺细胞混合移植可提高成肌细胞移植后的融合性。Objective To study the fusion of transplanted myoblast with host muscle cells and the expression of dystrophin in the muscle of mdx mice model for Duchenne muscular dystrophy（DMD） after local co-transplantation of myoblasts and schwann cells.Methods Myoblasts and schwann cells were prepared with gastrocnemius muscle taken from newborn C57BL/10 mice.Twenty four mdx mice were divided into two groups,one group received the injection of myoblasts into gastrocnemius muscle of left behind limb of mdx mice,,another group received the cotransplanation of schwann cells and myoblasts in a ratio of 1：10.The cells were transplanted to host mice once a week for four times.The mdx mice were sacrificed one month later,the expression of dystrophin in gastrocnemius muscle was measured,and the pathological and immunenohistochemistry exams were perfomed.Results The IOD of dystrophin of left gastrocnemius in co-transplantation group and myoblast transplantation group were 6342429.4167±925414.20068 and 5295615.7500±1243283.86373,respectively.The difference showed statistical significance,which indicated that the expression of dystrophin in co-transplantation group was higher than that of myoblast transplantation group.Conclusion local co-transplantation of myoblast and schwann cell can improve the myobalst’s fusion after transplantation.

关 键 词：成肌细胞 雪旺细胞 移植 抗肌萎缩蛋白 

分 类 号：R746[医药卫生—神经病学与精神病学]