微波辅助碱法提取匙吻鲟软骨蛋白-多糖复合物的工艺  

Extraction technology optimization of microwave-assisted alkali for protein-polysaccharide complex from Polyodon spathula cartilage

作　　者：丁俊胄[1,2,3] 沈硕[1,3] 熊善柏[1,2,3] 赵思明[1,2,3] 黄琪琳[1,2,3] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]国家大宗淡水鱼加工技术研发分中心,湖北武汉430070 [3]湖北省水产品加工工程技术研究中心,湖北武汉430070

出　　处：《水产学报》2011年第1期139-144,共6页Journal of Fisheries of China

基　　金：国家科技支撑计划(2006BAD30B01)

摘　　要：以匙吻鲟软骨为材料,利用微波辅助稀碱法提取软骨中的蛋白-多糖复合物,在对碱浓度、微波功率、微波时间和液料比进行单因素试验的基础上设计正交试验,优化提取条件。结果表明,在液料比为15∶1(V/W),碱浓度为0.9%,微波功率为360 W,微波处理为9 min时,得率达到最优值15.72%。与常规碱法提取相比,微波辅助提取不仅降低了碱用量,更加环保,而且缩短了提取时间,提高了得率。Polyodon spathula cartilage as the cartilage tissue mainly contains two kinds of functional components： protein and polysaccharide,which possess anti-tumor,anti-aging effects and so on.The extraction,structure as well as activities of polysaccharides from the cartilage were investigated extensively and deeply.However,the extract technology for protein-polysaccharide complex has less been reported compared with polysaccharides.Therefore,the present work uses Polyodon spathula cartilage as material,and focuses on the process of protein-polysaccharide complex extracted by microwave-assisted alkali.The effects of alkali concentration,microwave power,microwave time and the ratio of liquid to material on the extract yield of protein-polysaccharide complex were studied by single factor analysis.On the basis of single factor analysis,the orthogonal test was performed to optimize the extract technology of microwave-assisted alkali.The results indicated that the optimum conditions of protein-polysaccharide complex from Polyodon spathula cartilage were cartilage with the ratio of liquid to material（15 ∶ 1,V /W）,was extracted by 0.9% alkali solution,and was synchronously treated with 360 W microwave power for 6 min to obtain extract yield of 15.72%.The extraction technology of the microwave-assisted alkali can reduce the consumption of alkali,shorten the extraction time,and enhance the extract yield of cartilage.This work is favorable to further purification of proteins and polysaccharide component from Polyodon spathula cartilage,as well as to development of new health food and medicinal function products by providing the technical basis.

关 键 词：匙吻鲟 软骨 微波辅助 蛋白-多糖复合物 提取 

分 类 号：TS201.1[轻工技术与工程—食品科学]