杨梅渣黄酮类化合物提取及其抗氧化活性研究  

Research on the extraction and anti-oxidation of flavonoids in Myrica rubra marc

作　　者：陈学丽[1] 叶立斌[1] 励建荣[1] 孙金才 陈卫[1] 许庆炎 Zhou K. 

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310035 [2]浙江海通食品集团,浙江慈溪315300 [3]Department of Food Science & Technology,Virginia Polytechnic Institute and State University,Blacksburg Virginia 24061

出　　处：《食品工业科技》2011年第2期85-87,91,共4页Science and Technology of Food Industry

基　　金：“十一五”国家科技支撑计划重点项目(2006BAD27B03);宁波市农业攻关国际科技合作项目(2008C10042)

摘　　要：杨梅是我国的特产水果,年产量约为100万t,其中杨梅渣约占果实总重的10%。目前以杨梅为资源的食品工业已初具规模,然而在杨梅深加工过程中,杨梅渣都作为废物进行处理,对其开发利用仍然一片空白。基于此,以杨梅渣为研究对象,对杨梅渣中黄酮类化合物进行提取、浓缩、萃取,分成四个级分(石油醚相、乙酸乙酯相、正丁醇相和水相);用DPPH法、Fenton反应法和还原能力评价了四个不同级分的杨梅渣提取物的体外抗氧化能力。结果表明,杨梅渣粗提物正丁醇相和乙酸乙酯相具有较强的清除DPPH自由基能力,其半抑制浓度(IC50)分别为30、40μg/mL,分别是维生素C(IC50=110μg/mL)的3.67、2.75倍。Myrica rubra is a specific fruit species in China,annual production of which is about 1 million tons,and Myrica rubra marc accounts for 10% of total weight of the fruit. At present,the food industry based on Myrica rubra resources has a certain scale,while the utilization of Myrica rubra marc is still blank. The fractions were acquired from Myrica rubra marc by a series of steps including extraction and concentration,followed by extraction with petroleum ether,ethyl acetate,n-butanol. The anti-oxidative activities of the extracts were evaluated in vitro,including DPPH radical scavenging activity,Fenton action and reducing power methods. The results showed that n-butanol fraction and ethyl acetate fraction of Myrica rubra marc crude extracts exhibited strong scavenging abilities for DPPH free radical,their half-inhibitory concentration（IC50）were 30μg/mL and 40μg/mL,respectively,and were approximately 3.67 times and 2.75 times as much as that of Vitamin C（110μg/mL）.

关 键 词：杨梅渣 总黄酮 提取 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]