一株产虾青素的黄杆菌C_(F-60)的研究  

STUDIES ON A ASTAXANTHIN-PRODUCING C_(F-60) STRAIN OF FLAVOBATERIUM

作　　者：张亮[1] 朱湘民[2] 

机构地区：[1]清华大学生物技术系,北京100084 [2]中科院武汉病毒研究所,武汉430071

出　　处：《微生物学通报》1999年第5期332-335,共4页Microbiology China

摘　　要：从土壤中分离到一株黄杆菌（Ｆｌａｖｏｂａｃｔｅｒｉｕｍｓｐｐ）Ｃ(Ｆ－６０)，该菌的生长需Ｍｇ２＋存在，ＭｇＳＯ４·７Ｈ２Ｏ的最适浓度为０．２％；蛋白胨是该菌株生长的最好氮源，它不能利用无机氮。种龄超过９６ｈ的菌体不能在新鲜培养基中生长。经５４ｈ的２Ｌ恒化器发酵，生物量达６．８ｇ／Ｌ，色素产量为１０．６ｍｇ／Ｌ。该菌产生的类胡萝卜素成分简单，主要成分的含量为９０．３％，该成分经初步鉴定是分子结构中含有羰基和羟基的虾青素。A carotenoid-producing strain CF-60, was screened from the soil source and it was tentativelyclassified as Flavobacterium spp. The optimal growth conditions were studied, discovering that the CF-60 strainrequired Mg2+ in the medium, and the inoculate ages played an important role in it s growth. When cultivatedin 2 liter fermentor for 54h, the biomass and the pigment yield maximally reached 6.8g (dry cell) / liter and10.6mg / liter, respectively. The major component of the carotenoid was primarily identified as astaxanthin, whichwas possessed of a powerful function of antioxidation.

关 键 词：黄杆菌 类胡萝卜素 虾青素 发酵条件 色谱分离 

分 类 号：Q939.129[生物学—微生物学]