OPA-FMOC联用柱前衍生化法测定复方氨基酸注射液中氨基酸的含量  

Determination of amino acids in compound amino acid injection by pre-column OPA-FMOC derivatization

作　　者：陆明[1,2] 孙黛妮[1] 汪杨[1] 邵泓[1] 

机构地区：[1]上海市食品药品检验所,上海201203 [2]第二军医大学,上海200433

出　　处：《药物分析杂志》2010年第12期2323-2327,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立氨基酸含量的柱前衍生化HPLC的定量测定方法。方法:采用OPA-FMOC柱前衍生化法测定复方氨基酸注射液中各个氨基酸的含量并用内、外标法进行了方法比较。结果:表明2种方法在0.025～2.5μmol.mL-1范围内线性关系良好,相关系数在0.9994～1.000之间,平均回收率96.2%～103.6%,RSD<1.4%(n=6),重复性RSD为0.08%～2.82%(n=6),最低定量限0.2～1.1ng。结论:该方法准确、灵敏、简便。内、外标法均适用于各个氨基酸的含量测定。Objective：To develop an HPLC method for the determination of amino acids by pre-column OPA-FMOC derivatization.Methods：The pre-column OPA-FMOC derivatization assay was used to quantify amino acids in compound amino acid injection and the method was compared by internal or external standards.Results：The calibration curve was linear in the range of 0.025-2.5 μmol·mL-1with correlation of 0.9994~1.000.The average recovery rate was in the range of 96.2%-103.6%,RSD1.37%（n=6）,the method precision RSD was in the range of 0.08%-2.82%（n=6）with the lower limit of quantification for 0.2 μg·mL-1.Conclusion：The method is accurate,sensitive and simple,both of the internal and external standards methods proved to be suitable for the quantification of every amino acids.

关 键 词：邻苯二甲醛(OPA) 9-芴基氯甲酸甲酯(FMOC) 柱前衍生法 氨基酸 

分 类 号：R917[医药卫生—药物分析学]