Genistein增加顺铂诱导的SKOV-3细胞的凋亡与活性氧的关系  

Effect of Genistein on increasing apoptosis of drug-resistant ovarian cancer cell SKOV-3 induced by cisplatin

作　　者：魏燕[1] 辛晓燕[1] 黄艳红[1] 邵娟[1] 段海霞[1] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710032

出　　处：《现代生物医学进展》2010年第23期4446-4450,共5页Progress in Modern Biomedicine

摘　　要：目的:探讨Genistein增加顺铂诱导的耐药卵巢癌细胞SKOV-3凋亡的可能作用机制。方法:倒置相差显微镜下观察药物处理后细胞形态学的变化;MTT比色法检测不同药物处理后对SKOV-3细胞增殖的影响;流式细胞仪检测药物处理后细胞的凋亡情况;流式细胞仪和荧光显微镜检测细胞内活性氧(ROS)的水平。结果:10ug/ml的Genistein和2.5ug/ml的顺铂联用24h后,引起了细胞内ROS的增加,细胞的凋亡率也显著增高,与单用顺铂组相比差异有显著性(P<0.05);用NAC预处理细胞2h后,有效抑制了ROS的产生,并增加了细胞的活性,降低了细胞的凋亡率,与未加NAC组相比差异有显著性(P<0.05)。结论:Genistein增加顺铂诱导的耐药卵巢癌细胞SKOV-3的凋亡与细胞内ROS水平的升高有关,这可能是Genistein增加顺铂诱导的耐药卵巢癌细胞SKOV-3凋亡的作用机制之一。Objective：To investigate the mechanism of genistein combined with cisplatin-induced apoptosis in drug-resistant ovar-ian cancer cell SKOV-3.Methods：The morphological alterations of SKOV-3 cells were demonstrated by microscope.The growth in-hibitory effect of different drugs on SKOV-3 cells was detected by MTT assay.The apoptosis rate was determined by flow cytometry.The level of ROS was measured by both flow cytometry and Fluorescence Microscope.Results：After co-treatment of 10ug/ml genistein and 2.5ug/ml cisplatin for 24h,the level of ROS increased obviously,and the apoptotic rate was also significantly raised,as compared with cisplatin alone（P0.05）.Pretreatment with antioxidant NAC,ROS was effectively inhibited,cell viability was increased and the apoptotic rate was decrased.Conclusion：Genistein potentiated cisplatin-induced apoptosis in drug-resistant ovarian cancer cell SKOV-3 is related to the increased ROS.It may be one of the mechanisms of apoptosis induced by the combination in SKOV-3 cells.

关 键 词：GENISTEIN 卵巢癌 细胞凋亡 活性氧 

分 类 号：R737.31[医药卫生—肿瘤] Q254[医药卫生—临床医学]