固相萃取-气相色谱法测定鱼肉中硫丹残留量  

Determination of Endosulfan pesticide residues in fish by solid-phase extraction and capillary gas chromatography

作　　者：覃玲[1] 罗光毅[1] 蔡超海[1] 

机构地区：[1]广东省茂名市疾病预防控制中心,广东茂名525000

出　　处：《中国卫生检验杂志》2010年第12期3193-3194,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:用固相萃取-毛细管气相色谱分析方法测定鱼肉中的硫丹残留量。方法:样品以乙腈溶剂超声提取、Florisil固相萃取小柱净化处理后,采用DB-5毛细管气相色谱柱分离样品,电子捕获检测器检测。结果:硫丹在0.005μg/ml～0.100μg/ml范围内有良好的线性关系,相关系数为0.999。平均回收率为79.3%～90.3%,RSD为1.4%～3.9%。最小检测量为0.5μg/kg。结论:本法简便、灵敏、准确、重现性好,可用于鱼肉中硫丹残留量测定。Objective：To establish solid-phase extraction and capillary gas chromatography for the determining Endosulfan in fish.Methods：The Endosulfan pesticide residues were extracted from samples with solvents by ultrasonic and cleaned up by Florisil solidphase extraction column.Then,the extract was separated by capillary column and detected by electron capture detector.Results：There was good 1iner relationover the range of0.005 μg/L～0.100 μg/L.The average recoveries and RSD were in the range of 79.3%～90.5%and 1.4%～3.9% at three spiked mixed organOch1orine pesticides levels,respectively.The limits of detection were 0.5 μg/kg.Conclusion： This method is effective,fast,accurate,repeatable and suitable for analysis on Endosulfan residuesin tradition medicines materials.

关 键 词：鱼肉 硫丹 固相萃取 气相色谱 

分 类 号：TS207.53[轻工技术与工程—食品科学]