PCR检测牛乳中大肠杆菌基因组DNA的提取方法  

Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

作　　者：宋宏新[1] 邹联柱[1] 马冬[2] 薛海燕[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021 [2]华北煤炭医学院,河北唐山063000

出　　处：《食品科学》2010年第24期308-310,共3页Food Science

基　　金：陕西省科学技术厅农业攻关项目(2009K02-09);陕西省教育厅产业化培育项目(09JC19)

摘　　要：为获得一种适于牛乳样品大肠杆菌PCR检测的基因组DNA提取方法,对饱和酚法、CTAB法、试剂盒法及溶剂裂解法等4种提取方法加以比较,通过考察DNA的纯度、定量分析以及PCR分析,确定一套有效、快速、适合牛乳中提取大肠杆菌的基因组DNA方法——溶剂热裂解法。结果显示该方法提取的DNA模板,适于PCR扩增大肠杆菌DNA,可以用于牛乳中的大肠杆菌检测。In order to obtain an appropriate sample for PCR detection, four extraction methods including saturated phenol method, CTAB method, kit method and solvent pyrolysis method for E. coli genomic DNA in milk samples were compared. Through evaluating the purity, quantitative analysis and PCR analysis of genomic DNA extracted by four methods, an effective and fast solvent-thermal pyrolysis method was suitable for the extraction of E. coli genomic DNA in milk. The genomic DNA extracted by this method used as the template is suitable for PCR amplification of E. coli DNA, which can be used for the detection of E. coli in milk.

关 键 词：乳品 大肠杆菌 基因组总DNA 聚合酶链式反应 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]