胶体金试纸条快速检测食品中磺胺二甲基嘧啶残留  

Rapid Detection of Sulfadimidine Residue in Food by Colloidal Gold Strips

作　　者：林晓丽[1] 饶辉 熊勇华[1] 陈媛 刘文娟 赖卫华[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西省兽药饲料监察所,江西南昌330029 [3]江西中德生物工程有限公司,江西南昌330029

出　　处：《食品科学》2010年第24期366-369,共4页Food Science

基　　金：教育部"长江学者和创新团队发展计划"资助项目(IRT0540);南昌市重大产业化项目(2009)

摘　　要：目的:旨在建立一种快速、简便的针对磺胺二甲基嘧啶残留的检测方法。方法:应用竞争抑制免疫层析原理,研制磺胺二甲基嘧啶胶体金试纸条。结果:该试纸条检测限为10ng/mL、检测时间5min,与其他磺胺类药物交叉反应小,可用于多种食品样本的检测,且样本检测限为100μg/kg。结论:该方法操作简便、灵敏度高且特异性强,可实现对食品样品中磺胺二甲基嘧啶残留的快速检测。Objective： To establish a rapid and convenient detection method for sulfadimidine residue in food. Methods： The colloidal gold strip for specific sulfadimidine was developed successfully on the basis of competitive inhibition immunochromatography mechanism. Results： The detection limit of the colloidal gold strip was 10 ng/mL for sulfadimidine within less than 5 min, which also had low cross-reactivity with other sulfonamides. The colloidal gold strip could be used in rapid detection of sulfadimidine residue in many kinds of food samples with detection limit of 100 μg/kg. Conclusion： This method is characteristics of simplicity, high sensitivity and specificity to achieve the rapid detection of sulfadimidine residue in food.

关 键 词：磺胺二甲基嘧啶 胶体金 试纸条 快速检测 

分 类 号：TS207.7[轻工技术与工程—食品科学]