耐氧性双歧杆菌Bifidobacterium animalis subsp. lactis Qq08的生物学特性研究  

Studies on Some Physiological and Biochemical Characteristics of Aerotolerant Bifidobacterium Animalis Subsp.Lactis Qq08

作　　者：李青青[1,2] 傅伟[1] 陈路清[1] 朱东升[1] 陈启和[1] 阮晖[1] 何国庆[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029 [2]浙江省医学科学院保健食品所,杭州310000

出　　处：《中国食品学报》2010年第6期59-63,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省科技计划项目(2006C12095)

摘　　要：利用体外试验测定耐氧性双歧杆菌(Bifidobacterium animalis subsp.lactis Qq08)的耐酸、耐胆汁特性;同时测定其对胆固醇的降解能力和对结肠癌腺细胞Caco-2的黏附性能。结果表明,该菌在pH 2.0条件下培养4 h,仍有84.38%的存活率;用1.0%胆汁培养6 h,该菌的存活率在90%以上。该菌对胆固醇的降解力为53.8%,对结肠癌腺细胞Caco-2的黏附数为(8.57±3.74)个/细胞。该菌可作为益生菌制剂和功能性乳制品的潜在菌株。In order to lay a foundation for its production and utilization in probiotic products,the functional properties of Bifidobacterium animalis subsp.lactis Qq08 were studied,namely the resistance against low pH and high bile concentration,cholesterol-reducing ability and adhesion properties were tested by in vitro studies.Results indicated that strain Qq08 showed high tolerance to low pH,giving a survival rate of 84.38%,at pH 2 after 4 h incubation,and high tolerance to bile,more than 90% after 6 h incubation at 0.01 g/mL bile concentration.The cholesterol-reducing rate of strain Qq08 was 53.8%.The adhesion ability number to Caco-2 was（8.57±3.74）CFU/cell.The strain Qq08 performed excellently in probiotic characteristics and it can be used as potential strain in development of probiotics and functional dairy products.

关 键 词：耐氧性 双歧杆菌 生物学特性 胆固醇 

分 类 号：R371[医药卫生—病原生物学]