重组谷氨酰胺转胺酶基因的原核表达研究  

Study on Prokaryotic Expression of Recombined MTG Gene's

作　　者：何冬兰[1] 张莹[1] 彭宝玉[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2010年第4期32-36,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：微生物与生物转化中南民族大学重点实验室项目(XJ509002)

摘　　要：采用基因重组手段构建了含重组谷氨酰胺转胺酶基因的菌株E.coli BL21/pET30α-MTG(DE3).研究了诱导剂浓度、诱导时间、温度和转数等对重组体表达的影响,确定了融合蛋白pET30α-MTG的最佳表达条件.结果表明:当摇菌转速250 r/min,IPTG终浓度1.0 mmol/L,温度37℃时,诱导培养4 h可得最大表达量.A prokaryotic expression vector-E. coli BL21/pET30α-MTG（DE3）was constructed and the effects of IPTG concentration, reducing time, temperature and shaking conditions on the expression of the recombinant pET30α-MTG were studied. The results showed that the optional condition for the expression of recombined MTG Gene were as follows： IPTG final concentration 1. 0 mmol/L, rotation speed 250 r/min, inducing temperature 37℃and inducing time 4 h.

关 键 词：微生物谷氨酰胺转胺酶 重组 基因 表达 

分 类 号：R344.31[医药卫生—基础医学]