顺铂载入碳纳米管内增加其抗肿瘤性的研究  

Study on the enhancement of anticancer effects of cisplatin by single-walled carbon nanotubes with larger diameters

作　　者：彭芸花[1,2] 金玉[2] 王兴秀 孙少伯 

机构地区：[1]兰州大学基础医学院,兰州730000 [2]兰州大学第一医院妇产科,兰州730000 [3]兰州石化总医院,兰州730000 [4]甘肃省中医学院,兰州730000

出　　处：《中国临床药理学杂志》2010年第12期920-922,953,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的观察大孔径碳纳米管(SWNTs)做为载体以增加顺铂抗肿瘤活性的模式及机制。方法用毛细现象,将碘化丙啶(PI)和顺铂装载进入纳米管管腔,内载顺铂进入宫颈癌细胞,并在细胞内释放顺铂;用荧光分光光度计及紫外分光光度计,观察PI及顺铂在细胞外和细胞内释放模式,并通过流式细胞技术及MTT法,检测顺铂对宫颈癌细胞增殖活性的抑制。结果顺铂在碳纳米管的运载下,可较易进入细胞内,从而使细胞内药物浓度增加,其模式与PI的转载模式相同,且在顺铂浓度较低时,就可对Hela细胞产生明显的抑制作用。结论 SWNTs内载抗癌药物可逆转肿瘤耐药,增加药物抗肿瘤作用。Objective To study the extracellular as well as the intracellular drug release mode,the drug delivery ability,and the anticancer potential of single-walled carbon nanotubes（SWNTs）with larger diameters（20-50 nm）loaded with cisplatin or propidium iodide（PI）in cervical cancer cells.Methods Determination of extracellular and intracellar PI and cisplatin release mode of drug-loaded SWNTs by UV spectrophotometer and fluorospectrophotometer.To observe the cytotoxic effect of cisplatin-loaded SWNTs on the proliferation of Hela cells by MTT assay and flow cytometry detection.Results Our findings was demonstrated that SWNTs with larger diameters（20-50 nm）has good membrane penetration and drug delivery abilities.Cisplatin-loaded SWNTs has a similar extracellular drug release mode to PI-loaded SWNTs,and cisplatin-loaded SWNTs can enhance the anticancer effects of cisplatin in cervical cancer cells in a concentration-dependent manner.Conclusion Our study was suggested that SWNTs with larger diameters（20-50 nm）is a good carrier for fluorescent reagents and therapeutic agents,and cisplatin-loaded SWNTs with larger diameters（20-50 nm）is a promising anti-cancer agent for cervical cancers.

关 键 词：大孔径纳米管 顺铂 HELA细胞 增殖活性 

分 类 号：R737.33[医药卫生—肿瘤] R979.1[医药卫生—临床医学]