Optimization of Fermentation Conditions of Acid Resistant α-amylase Producing Strain  

耐酸性α-淀粉酶产生菌的发酵条件优化(摘要)(英文)

作　　者：刘雅琴[1] 乌日娜[1] 段金华[1] 

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《Agricultural Science & Technology》2010年第8期73-76,共4页农业科学与技术（英文版）

基　　金：Supported by the Project Funded by Biotechnology Key Laboratory of Fermentation and Brewing Engineering of State Ethnic Affairs Commission (2008SY011)~~

摘　　要：[Objective] The aim was to optimize the fermentation conditions of acid resistant α-amylase producing strain. [Method] Based on the selection of an acid resistant α-amylase producing strain,the fermentation conditions including C,N contents and initial pH of culture medium,seed age,inoculum size,rotation speed of shake flask and fermentation temperature were optimized. [Result] The optimum fermentation conditions for acid resistant α-amylase producing strain were：seed age 14 h,inoculum size 8%,initial pH 5.5,fermentation temperature 35 ℃,rotation speed 150 r/min,the volume of inoculum broth 25 ml,C content 1.0% and N content 0.5%. [Conclusion] Under the optimum fermentation conditions,α-amylase activity reached 31.4 U/ml,which was 65.3 % higher than that before optimization.[目的]优化耐酸性α-淀粉酶产生菌的发酵条件。[方法]在筛选出的耐酸性α-淀粉酶产生菌的基础上,对其培养基C、N含量、接种龄、接种量、初始pH、摇瓶转速及温度等发酵条件进行优化。[结果]耐酸性α-淀粉酶产生菌最佳发酵条件为接种龄14h,接种量8%,初始pH值5.5,发酵温度35℃,转速150r/min,接种菌液量25ml,培养基中C、N的含量分别为1.0%、0.5%。[结论]在优化条件下,酶活力达到31.4U/ml,比未优化时提高了65.3%。

关 键 词：ALPHA-AMYLASE Enzyme activity FERMENTATION Optimization 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]