Isolation and Identification of Triazophos-degrading Bacillus subtilis str. C-Y106 and Its Degradation Characteristics  

三唑磷降解菌Bacillus subtilis str. C-Y106的分离、鉴定及其降解特性研究(摘要)(英文)

作　　者：陈勇辉[1,2] 付桂明[1,2,3] 万茵[1,2] 韩蓓[4] 罗阳帆[1,2,3] 王建涛[1,2,4] 李红歌[1,2,4] 陈建芳[1,2,3] 柴建新[1,2,3] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学生命科学与食品工程学院,江西南昌330031 [3]南昌大学中德食品工程中心,江西南昌330047 [4]西安交通大学医学院公共卫生系,陕西西安710061

出　　处：《Agricultural Science & Technology》2010年第8期77-80,113,共5页农业科学与技术（英文版）

摘　　要：[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.[目的]筛选能高效降解三唑磷的菌株,并研究其降解特性。[方法]从三唑磷生产厂的曝气池活性污泥中分离到1株三唑磷降解菌C-Y106,并根据C-Y106的形态、生理生化特征和16S rRNA同源性序列分析进行了菌株鉴定,同时研究了不同营养的培养基对C-Y106降解三唑磷速率的影响。[结果]经鉴定,C-Y106为枯草芽孢杆菌。C-Y106能以三唑磷为唯一碳源、唯一氮源、唯一磷源生长,其中以三唑磷为唯一磷源时其降解速率最大,且在31℃、初始pH8.0、150r/min条件下,培养60h后,三唑磷降解率为76.8%。[结论]该研究为三唑磷降解酶的分离纯化提供了理论依据。

关 键 词：Triazophos-degrading Bacillus subtilis str.C-Y106 Degradation characteristics 

分 类 号：X172[环境科学与工程—环境科学]