凝胶渗透色谱-高效液相色谱法对辣椒酱中14种邻苯二甲酸酯的同时检测  

Simultaneous Determination of 14 Phthalate Esters in Chili Sauce by GPC-HPLC

作　　者：张会军[1] 张敬轩[1] 李挥[1] 张岩[1] 

机构地区：[1]河北省食品质量监督检验研究院,河北省食品安全重点实验室,河北石家庄050051

出　　处：《食品科学》2011年第4期152-154,共3页Food Science

基　　金：河北省科技支撑计划项目(09227114D;09227134D)

摘　　要：建立辣椒酱中14种邻苯二甲酸酯类环境激素的高效液相色谱-二极管阵列检测器(HPLC-DAD)检测方法。样品通过石油醚超声提取,GPC净化,氮吹浓缩后,以乙腈-水为流动相,经Warters-C18(250mm×4.6mm,5μm)液相色谱柱分离,进行HPLC-DAD分析。方法线性相关系数r>0.998,检出限为0.25～2.0mg/kg,在10mg/kg添加水平时样品加标回收率为85.7%～100.8%,RSD为0.37%～4.57%。该方法灵敏、快速、高通量,可用于14种邻苯二甲酸酯雌激素的测定。A GPC-HPLC-DAD method was established for the simultaneous determination of 14 kinds of phthalate esters （PAEs） in chili sauce. Samples were extracted with petroleum ether in an ultrasonic field,followed by clean-up by gel permeation chromatography and concentration by nitrogen gas blowing. The chromatographic separation was achieved on Waters-C18 column （250 mm × 4.6 mm,5μm） using an acetonitrile-water mobile phase. A HPLC system coupled with a diode array detector （DAD） was used to analyze the analytes. The developed analytical method exhibited a linear correlation coefficient of more than 0.998 and detection limits ranging from 0.25 to 2.0 mg/kg. Spike recovery rates between 85.7% and 100.8% were obtained,with relative standard deviations （RSD） of 0.37%-4.57%. Therefore,this method is a sensitive,rapid high throughout method and has good suitability to be used to analysis 14 kinds of PAEs in chili sauce.

关 键 词：凝胶净化 高效液相色谱 辣椒酱 邻苯二甲酸酯 

分 类 号：R994.6[医药卫生—毒理学]