超高效液相色谱法同时测定饮料中5种人工合成色素  

Determination of Five Synthetic Pigments in Beverage by Ultra Performance Liquid Chromatography

作　　者：张婉[1] 王覃[1] 杜宁[1] 周悦[1] 李津廷[1] 张经华[1] 

机构地区：[1]北京市理化分析测试中心,北京100089

出　　处：《食品科学》2011年第4期177-180,共4页Food Science

基　　金：北京市科学技术研究院创新团队项目(IG200801N/C2)

摘　　要：目的:建立超高效液相色谱同时测定饮料中5种人工合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝)的检测方法。方法:样品采用聚酰胺吸附法提取色素,通过WatersACQUITYUPLCTMBEHC18反相色谱柱分离,以甲醇-0.02mol/L乙酸铵缓冲溶液为流动相进行梯度洗脱,于254nm和629nm波长处检测。结果:5种人工合成色素在5.5min内完成分离,3～80mg/L范围内呈良好线性关系(r>0.9995),检出限为0.03～0.10mg/L,平均回收率为97.4%～107.3%,RSD为0.22%～3.20%。结论:该方法简便、快速、分离效果好、灵敏度高,适用于饮料中人工合成色素的测定。Objective：To develop an ultra performance liquid chromatography （UPLC） method for the determination of five synthetic pigments such as tatrazine,amaranth,carmine,sunset yellow and brilliant blue in beverage. Methods：Synthetic pigments in beverage were extracted by polyamide adsorption method,separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate,and detected at 254 nm and 629 nm. Results：The five synthetic pigments were separated in 5.5 min. The UPLC method exhibited excellent linearity over a range of 3-80 mg/L （r 0.9995）. The detection limit,recovery rate and relative standard deviation （RSD） of this method were 0.03-0.10 mg/L,97.4%-107.3%,and 0.22%-3.20%,respectively. Conclusion：The analytical method is simple,accurate,sensitive and suitable for the determination of pigments in beverage.

关 键 词：超高效液相色谱法 人工合成色素 饮料 

分 类 号：O657.63[理学—分析化学]