花盖红菇菌丝体提取物的体外功效及组分分析  

Bioactivity and Chemical Components of Mycelial Extracts of Russula cyanoxantha

作　　者：袁云辉[1] 张树斌[1] 姚钢乾[2] 赵俊霞[3] 王立安[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]河北旅游职业学院生物工程系,河北承德067000 [3]河北医科大学基础医学院,河北石家庄050017

出　　处：《食品科学》2011年第3期223-227,共5页Food Science

基　　金：河北省自然科学基金项目(C2008000155);河北省教育厅重点研究计划项目(ZH2007107)

摘　　要：采用摇瓶发酵获得花盖红菇菌丝体,用石油醚、乙酸乙酯、乙醇和水提取菌丝体活性物质,得到不同的提取物。采用清除DPPH自由基实验和抑制小鼠肝细胞脂质过氧化活性实验检测各提取物的抗氧化活性。结果显示:乙酸乙酯提取物和乙醇提取物均具有较高的抗氧化活性,其中以乙醇提取物活性最高。MTT法检测各提取物对H446肺癌细胞增殖的抑制作用,结果表明:石油醚提取物、乙酸乙酯提取物及乙醇提取物均有较高的抗肿瘤活性。采用色谱技术对乙醇提取物进行分离纯化,得到3个单一组分,分别命名为组分Ⅰ、Ⅱ、Ⅲ;经结构鉴定组分Ⅰ为1-氮杂[4,2,0]双环-4-烯-8-酮。Russula cyanoxantha was cultured using liquid fermentation medium. Mycelia ofRussula cyanoxantha were subjected to extraction by petroleum ether （PE）, ethyl acetate （EA） and ethanol, respectively. Antioxidant activities of different extraction fractions were evaluated by scavenging capacity on DPPH free radicals and inhibitory rate of lipid peroxidation. Results indicated that EA-soluble extract and ethanol-soluble extract had better antioxidant activities and the ethanol-soluble extract revealed the highest antioxidant capability. MTT assay indicated that PE, EA and ethanol extracts had obvious inhibition effects on the proliferation of H446 cancer cells, suggesting the anti-tumor activities of these extracts. The compositions of ethanol extract from mycelia of Russula cyanoxantha were analyzed. Three fractions were achieved by chromatographic separation as Fraction I, II and III, respectively. Fraction I was identified as 1-aza-bicyclo [4.2.0] oct-4-en-8-one by IR, FAB-MS, ^1H NMR, ^13C NMR, HSQC and HMBC methods.

关 键 词：花盖红菇 菌丝体 乙醇提取物 抗氧化 抗肿瘤 

分 类 号：Q939.5[生物学—微生物学]