低能N^+注入对α-淀粉酶二级结构及其活性的影响  

Effect of low energy N^+ ion beam implantation on the secondary structure and activity of α-amylase

作　　者：许强[1] 杨体强[2,3] 王苗[4] 

机构地区：[1]宝鸡文理学院物理与信息技术系,宝鸡721016 [2]内蒙古自治区离子束生物工程重点实验室,呼和浩特010021 [3]内蒙古大学物理科学与技术学院,呼和浩特010021 [4]宝鸡文理学院图书馆,宝鸡721016

出　　处：《核技术》2011年第2期147-150,共4页Nuclear Techniques

基　　金：陕西省科学技术研究计划(2010K01-078)项目;陕西省教育厅自然科学专项基金(08JK214);宝鸡文理学院重点项目(ZK1015)

摘　　要：本文介绍利用圆二色(CD)光谱法研究20 keV N+注入对α-淀粉酶二级结构的影响,测定其CD光谱,分析了酶活性变化的原因。结果表明:在1015–1016/cm2范围,不同注量的低能N+注入对α-淀粉酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量的影响程度不同。低能N+注入使α-淀粉酶活性发生改变,酶活性的改变与其空间结构的变化密不可分。Samples of the α-amylase were treated with 10^15-10^16 ions/cm2 of 20 keV N＋. The secondary structure of the ion-implanted α-amylase was studied by circular dichroism, and activity change of the α-amylase was analyzed. The results showed that in the dose range under investigation, the N＋ implantation affected the relative contents of α-helix, β-sheet, β-turn and random coil of the α-amylase, but no significant differences were found between the samples implanted to different doses. The activity of the N＋-implanted α-amylase changed, with a relationship between the activity increase and the conformation change.

关 键 词：离子注入 Α-淀粉酶 圆二色光谱 二级结构 酶活性 

分 类 号：O657.39[理学—分析化学] Q683[理学—化学]