高效液相色谱法同时测定香兰素与邻位香兰素  

Simultaneous Determination of Vanillin and o-Vanillin by High Performance Liquid Chromatography

作　　者：金海涛[1] 马健瑜[1] 王晓珣[1] 陈星[1] 任丽艳[1] 关瑾[1] 李云[1] 

机构地区：[1]沈阳化工大学应用化学学院,辽宁沈阳110142

出　　处：《分析测试学报》2011年第2期222-224,228,共4页Journal of Instrumental Analysis

基　　金：辽宁省自然基金资助项目(20090655);辽宁省教育厅2008年度科研计划项目(2008S184)

摘　　要：建立了同时测定香兰素和邻位香兰素的高效液相色谱法。考察了流动相组成、柱温等因素对分析效率的影响。在流动相为5％乙酸-乙腈（60：40，体积比），流速1．0mL／min，柱温25℃的优化条件下，香兰素和邻位香兰素可在5min内实现分离。测定结果表明，香兰素和邻位香兰素在10～240mg／L范围内线性关系良好，相关系数均为0．9997；方法的检出限（S／N=3）分别为0．06、0．02mg／L；样品的加标回收率为99％～102％，相对标准偏差为0．2％～0．6％。该方法操作简单、快速，用于实际样品的分析，结果满意。A high performance liquid chromatographic method was developed for the simultaneous separation and determination of vanillin and o-vanillin. Influences of mobile phase composition and column temperature on the separation were investigated. Under the condition of 5% acetic acid - acetonitrile （60 ： 40, by volume） as mobile phase at a flow rate of 1.0 mL/min and 25 ℃, the vanillin and o-vanillin were separated in less than 5 min. The calibration curves of vanillin and o-vanillin showed good linearities in the range of 10 - 240 mg/L with both correlation coefficients of 0. 999 7. The limits of detection（ S/N = 3 ） for vanillin and o-vanillin were 0.06 mg/L and 0.02 mg/L, respectively. The average recoveries at three spiked levels were in the range of 99% -102% with relative standard deviations of 0. 2% - 0.6%. The method was successfully used for the determination of vanillin and o-vanillin in real samples with satisfactory results.

关 键 词：高效液相色谱法 香兰素 邻位香兰素 同分异构体 

分 类 号：O657.72[理学—分析化学] S816.74[理学—化学]