一种快速定性和定量检测发酵乳中L.fermentum的方法  

A rapid qualitative and quantitative detection method of L.fermentum in the fermented milk

作　　者：包秋华[1] 陈永福[1] 张家超[1] 孙志宏[1] 包艳[1] 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2011年第2期59-61,共3页China Dairy Industry

基　　金：973计划前期研究专项(2010CB134502);教育部创新团队发展计划(IRT0967);国家自然科学基金项目(No.30760156)

摘　　要：通过比对发酵乳杆菌(L.fermentum)与其他乳酸菌的16S rRNA基因序列的异同,设计出L.fermentum的种属特异性引物,应用此引物进行种属特异性PCR和实时荧光定量PCR反应,通过琼脂糖凝胶电泳图谱和实时荧光定量PCR图谱分析,建立一种快速检测发酵乳中L.fermentum的定性和定量测定方法。The differences in 16S rRNA gene region between L. fermentum and other Lactic acid bacteria were compared. The species specificity primer of L. fermentum was designed and used to carry out species-specific PCR. assay and fluorescent quantitative real-time PCR. Through analyzing the electrophoretic pattern and the curve of RT-PCR, a rapid qualitative and quantitative method for the detection of L. fermentum in the fermented milk was established.

关 键 词：乳酸菌 发酵乳杆菌 PCR 实时荧光定量PCR 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]